Microarray expression profiling identifies genes with altered expression in HDL-deficient mice
Microarray expression profiling identifies genes with altered expression in HDL-deficient mice
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DOI：
10.1101/gr.10.12.2022
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发表时间：
2000-12-01
期刊：
影响因子：
7
通讯作者：
Rubin, EM
中科院分区：
文献类型：
--
作者：
Callow, MJ;Dudoit, S;Rubin, EM
基于已知参与高密度脂蛋白（HDL）代谢的基因表达的严重改变可能影响其他基因表达的假设，我们筛选了一系列>5000个小鼠表达的序列标签，用于改变HDL血浆浓度显著降低的两个品系小鼠肝脏中的基因表达。将来自载脂蛋白Al（apoAl）基因敲除小鼠、清道夫受体BI（SR-BI）转基因小鼠和对照小鼠肝脏的标记cDNA与微阵列共杂交。双样本t统计被用来确定基因敲除或转基因小鼠与对照小鼠相比，表达水平改变的基因。在SR-BI组中，我们发现9个阵列元素代表至少5个基因，这些基因在调整后的P值< 0.05的基础上显著改变。在apoAl基因敲除组中，与对照组相比，代表4个基因的8个阵列元件发生了改变（校正后P < 0.05）。在SR-BI转基因中鉴定的几个基因表明改变了甾醇代谢和氧化过程。这些研究说明了使用多重测试方法来识别重复微阵列实验中表达改变的基因。
Based on the assumption that severe alterations in the expression of genes known to be involved in high-density lipoprotein (HDL) metabolism may affect the expression of other genes, we screened an array of >5000 mouse expressed sequence tags For altered gene expression in the livers of two lines of mice with dramatic decreases in HDL plasma concentrations. Labeled cDNA from livers of apolipoprotein Al (apoAl)-knockout mice, scavenger receptor BI (SR-BI) transgenic mice, and control mice were cohybridized to microarrays. Two-sample t statistics were used to identify genes with altered expression levels in the knockout or transgenic mice compared with control mice. In the SR-BI group we found nine array elements representing at least Five genes that were significantly altered on the basis of an adjusted P value < 0.05. In the apoAl-knockout group, eight array elements representing Four genes were altered compared with the control group (adjusted P < 0.05). Several of the genes identified in the SR-BI transgenic suggest altered sterol metabolism and oxidative processes. These studies illustrate the use of multiple-testing methods for the identification of genes with altered expression in replicated microarray experiments.