A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines
A transaminase-mediated aldol reaction and applications in cascades to styryl pyridines
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DOI：
10.1039/d3cy01370g
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发表时间：
2024-03-01
影响因子：
5
通讯作者：
Hailes，Helen C.
中科院分区：
文献类型：
--
作者：
Wang，Yu;Li，Yiwen;Hailes，Helen C.
转氨酶是公认的生物催化剂，由于其有益的可持续性、区域和立体选择性以及底物专一性，被用于化学合成。在这里，使用一种野生型紫罗兰色杆菌转氨酶(CvTAm)在酶的级联反应中发现了一种新的羟基苯乙烯基吡啶产物的形成。随后的研究证实，这是一个转氨酶介导的反应，表现出明显的醛缩酶活性。然后使用其他野生型转氨酶并通过形成CvTAm突变体来探索这种混杂酶反应机制。随后开发了一锅多步酶级联的应用，以生产一系列羟基苯乙烯基吡啶。
Transaminase enzymes are well established biocatalysts that are used in chemical synthesis due to their beneficial sustainability profile, regio- and stereoselectivity and substrate specificity. Here, the use of a wild-type Chromobacterium violaceum transaminase (CvTAm) in enzyme cascades revealed the formation of a novel hydroxystyryl pyridine product. Subsequent studies established it was a transaminase mediated reaction where it was exhibiting apparent aldolase reactivity. This promiscuous enzyme reaction mechanism was then explored using other wild-type transaminases and via the formation of CvTAm mutants. Application of one pot multi-step enzyme cascades was subsequently developed to produce a range of hydroxystyryl pyridines.