Online monitoring of microcarrier based fibroblast cultivations with in situ microscopy
Online monitoring of microcarrier based fibroblast cultivations with in situ microscopy
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DOI：
10.1002/bit.21523
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发表时间：
2008-01-01
影响因子：
3.8
通讯作者：
Scheper, Thomas
中科院分区：
文献类型：
--
作者：
Rudolph, Guido;Lindner, Patrick;Scheper, Thomas
动物细胞培养在生物技术中被广泛用于生产许多生物产品。原位显微镜直接从细胞悬浮液中获取图像，并从细胞浓度、细胞大小分布和细胞形态等方面对图像进行分析。它们的适用性已经被证明用于酵母和悬浮哺乳动物细胞培养。在这项工作中，原位显微镜被用来测量在培养过程中的微载体表面上的成纤维细胞的定植水平。对于该研究，鼠细胞系NIH-3 T3与Cytodex 1微载体组合使用。在配备有原位显微镜的5L搅拌罐生物反应器中进行培养。在整个培养时间内用原位显微镜连续获得图像（每个序列900个图像，平均每个序列7.5小时）。对于微载体分析，开发了基于神经网络的图像分析算法，并在显微镜分析软件中实现。
Animal cell culture is widely used in biotechnology for the production of many biological products. In situ microscopes acquire images directly from cell suspensions and analyze the images in matters of cell concentration, cell size distribution and cell morphology. Their applicability was already proven for yeast and suspended mammalian cell cultivations. In this work the in situ microscope was utilized to measure the level of colonization of fibroblasts on microcarrier surfaces during cultivation. For this study the murine cell line NIH-3T3 was used in combination with Cytodex 1 microcarriers. Cultivations were carried out in a 5 L stirred tank bioreactor equipped with the in situ microscope. Images were obtained sequentially with the in situ microscope over the whole cultivation time (900 images per sequence, 7.5 h per sequence on average). For the microcarrier analysis an image analysis algorithm based on a neural network was developed and implemented in the microscope analysis software.