Neuraminidase from Trypanosoma cruzi: analysis of enhanced expression of the enzyme in infectious forms.
Neuraminidase from Trypanosoma cruzi: analysis of enhanced expression of the enzyme in infectious forms.
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来自克氏锥虫的神经氨酸酶：分析该酶在感染形式中表达的增强。
DOI：
10.1073/pnas.84.23.8320
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发表时间：
1987
影响因子：
11.1
通讯作者：
So,M
中科院分区：
文献类型：
--
作者：
Harth,G;Haidaris,CG;So,M
我们从克氏锥虫Y株中纯化了神经氨酸酶（唾液酸酶，酰基神经氨酸水解酶，EC 3.2.1.18），并研究了该酶的发育调控作用。在寄生虫从非感染性的初马鞭毛虫转变为感染性的锥马鞭毛虫后，酶活性的检测量增加了10- 20倍。该酶是从大于5000倍的锥乳虫膜中纯化出来的，以明显的均匀性，并在变性条件下作为Mr 60,000的实体迁移。兔对变性蛋白产生的抗体识别感染期膜提取物中的神经氨酸酶，但不识别非感染期膜提取物中的神经氨酸酶。急性血吸虫病患者的血清也与神经氨酸酶有强烈反应。其他克氏T. cruzi菌株表现出相似的神经氨酸酶活性和诱导率。神经氨酸酶在锥乳线虫体内的传染性和增强表达的巧合表明，该酶是克氏锥虫的一个毒力因子。
We purified the neuraminidase (sialidase, acylneuraminyl hydrolase, EC 3.2.1.18) from the protozoan parasite Trypanosoma cruzi, strain Y, and examined the developmental regulation of the enzyme. The detectable amount of enzyme activity increased 10- to 20-fold upon conversion of the parasite from the noninfectious epimastigote form to the infectious trypomastigote form. The enzyme was purified from membranes of trypomastigotes greater than 5000-fold to apparent homogeneity and migrated as an entity of Mr 60,000 under denaturing conditions. Antibodies produced in rabbits against the denatured protein recognized the neuraminidase in membrane extracts from the infectious stage but not from the noninfectious stage. Sera from a patient with acute chagasic disease also reacted strongly with the neuraminidase. Other T. cruzi strains exhibited similar neuraminidase activities and induction rates. The coincidence of infectivity and enhanced expression of neuraminidase in trypomastigotes suggests that this enzyme constitutes a virulence factor in T. cruzi.