Inactivation of Vesicular Stomatitis Virus with Light-Activated Carbon Dots and Mechanistic Implications
Inactivation of Vesicular Stomatitis Virus with Light-Activated Carbon Dots and Mechanistic Implications
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DOI：
10.1021/acsabm.2c00153
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发表时间：
2022-07-07
影响因子：
4.7
通讯作者：
Yang, Liju
中科院分区：
文献类型：
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作者：
Adcock, Audrey F.;Wang, Ping;Yang, Liju
预防病毒传播是解决病毒感染传播的重要步骤。以有包膜水泡性口炎病毒（VSV）为模型，研究了碳量子点（CDots）的抗病毒功能。CDot使用具有通过低聚聚乙烯亚胺（PEI）的表面官能化-钝化的小碳纳米颗粒制备。结果表明，PEI-CDot容易被可见光激活，以在实验条件（病毒滴度、点浓度和处理时间）的各种组合下有效且高效地抑制VSV。光动力学诱导的病毒结构蛋白降解和基因组RNA降解，提示了导致病毒失活的机制来源。结果表明，CDots是一类很有前途的广谱病毒消毒剂。
The prevention of viral transmission is an important step to address the spread of viral infections. Using the enveloped vesicular stomatitis virus (VSV) as a model, this study explored the antiviral functions of the specifically designed and prepared carbon dots (CDots). The CDots were prepared using small carbon nanoparticles with surface functionalization- passivation by oligomeric polyethylenimine (PEI). The results indicated that the PEI-CDots were readily activated by visible light to effectively and efficiently inactivate VSVs under various combinations of experimental conditions (viral titer, dot concentration, and treatment time). The photodynamically induced viral structural protein degradation and genomic RNA degradation were observed, suggesting the mechanistic origins, leading to the inactivation of virus. The results suggested CDots as a class of promising broad-spectrum antiviral agents for disinfection of viruses.