Effects of sera from lupus patients on the glomerular endothelial fibrinolysis system.
Effects of sera from lupus patients on the glomerular endothelial fibrinolysis system.
复制标题
狼疮患者血清对肾小球内皮纤溶系统的影响。
DOI：
10.1111/ped.15099
复制
发表时间：
2022
影响因子：
1.4
通讯作者：
Tanaka H
中科院分区：
文献类型：
--
作者：
Sato R;Aizawa T;Imaizumi T;Tsugawa K;Kawaguchi S;Seya K;Terui K;Tanaka H
研究背景狼疮性肾炎的发病机制与肾小球细胞凝血纤溶系统的失调有关。然而，纤溶酶原激活物抑制剂-1（派-1）和组织型纤溶酶原激活物（tPA）在居民肾小球cells中的作用仍未确定。MethodsWe研究了派-1和tPA mRNA的表达在培养的正常人肾小球内皮细胞（GECs）与血清治疗系统性红斑狼疮（SLE）患者使用定量逆转录聚合酶链反应。我们确定了派-1/tPA mRNA表达与几个临床/实验室参数之间的关系。16例患者的血清（9例新发SLE患者和7例稳定SLE患者）被用于在study.ResultsPlasminogen activator inhibitor-1和tPA mRNA的表达显着高于GECs治疗与新发SLE患者的血清比其他组。派-1和tPA mRNA水平在用SLE患者血清处理的GEC中也显著相关。有趣的是，派-1和tPA mRNA在GECs的水平与血清C4水平呈负相关，与SLE疾病activity.ConclusionsThese结果表明，SLE患者血清可能激活肾小球纤溶系统，这可能是参与狼疮肾炎的发病机制。
BackgroundDysregulation of the coagulation fibrinolysis system in resident glomerular cells is associated with the pathogenesis of lupus nephritis. However, the role of plasminogen activator inhibitor‐1 (PAI‐1) and tissue plasminogen activator (tPA) in resident glomerular cells remains undetermined.MethodsWe examined the expression of PAI‐1 and tPA mRNA in cultured normal human glomerular endothelial cells (GECs) treated with serum from patients with systemic lupus erythematosus (SLE) using quantitative reverse transcription polymerase chain reactions. We determined the relationship between PAI‐1/tPA mRNA expression and several clinical/laboratory parameters. Serum from 16 patients (nine patients with new‐onset SLE and seven patients with stable SLE) was used in the study.ResultsPlasminogen activator inhibitor‐1 and tPA mRNA expression was significantly higher in GECs treated with serum of patients with new‐onset SLE than other groups. The PAI‐1 and tPA mRNA levels were also significantly correlated in GECs treated with serum from patients with SLE. Interestingly, both PAI‐1 and tPA mRNA levels in GECs were inversely correlated with serum C4 level and positively correlated with SLE disease activity.ConclusionsThese results suggest that serum from patients with SLE may activate the fibrinolysis system in glomerulus, which may be involved in the pathogenesis of lupus nephritis.