Alternative Splicing Substantially Diversifies the Transcriptome during Early Photomorphogenesis and Correlates with the Energy Availability in Arabidopsis
Alternative Splicing Substantially Diversifies the Transcriptome during Early Photomorphogenesis and Correlates with the Energy Availability in Arabidopsis
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DOI：
10.1105/tpc.16.00508
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发表时间：
2016-11-01
期刊：
影响因子：
11.6
通讯作者：
Wachter, Andreas
中科院分区：
文献类型：
--
作者：
Hartmann, Lisa;Drewe-Boss, Philipp;Wachter, Andreas
植物利用光作为能量和信息的来源来检测昼夜节律和季节变化。感知光环境的变化对于调节植物的新陈代谢和启动发育转变至关重要。在这里，我们分析了基因表达和选择性剪接（AS）的黄化幼苗经历光形态建成后，暴露于蓝色，红色或白色光的转录组范围内的变化。我们的分析揭示了大规模的转录组重编程，这反映在所有基因的20%的差异表达和数百个AS事件的变化上。对于超过60%的所有受调节的AS事件，光促进了一种可能的蛋白质编码变体的产生，而代价是具有无义介导的衰变触发特征的mRNA。因此，推定的剪接因子REDUCED RED-LIGHT RESPONSES IN B112 GREUND 1的AS，先前被鉴定为红光信号传导组分，在光下被转移到功能变体。候选AS事件的下游分析指出，光感受器信号传导仅在单色光中起作用，而在白色光中不起作用。此外，我们证明了类似的AS光暴露和外源性糖供应后的变化，与激酶信号转导的关键参与。我们建议，AS是一个整合点的信号转导途径，传感和传输信息的能量供应在植物中。
Plants use light as source of energy and information to detect diurnal rhythms and seasonal changes. Sensing changing light conditions is critical to adjust plant metabolism and to initiate developmental transitions. Here, we analyzed transcriptomewide alterations in gene expression and alternative splicing (AS) of etiolated seedlings undergoing photomorphogenesis upon exposure to blue, red, or white light. Our analysis revealed massive transcriptome reprogramming as reflected by differential expression of similar to 20% of all genes and changes in several hundred AS events. For more than 60% of all regulated AS events, light promoted the production of a presumably protein-coding variant at the expense of an mRNA with nonsense-mediated decay-triggering features. Accordingly, AS of the putative splicing factor REDUCED RED-LIGHT RESPONSES IN CRY1CRY2 BACKGROUND1, previously identified as a red light signaling component, was shifted to the functional variant under light. Downstream analyses of candidate AS events pointed at a role of photoreceptor signaling only in monochromatic but not in white light. Furthermore, we demonstrated similar AS changes upon light exposure and exogenous sugar supply, with a critical involvement of kinase signaling. We propose that AS is an integration point of signaling pathways that sense and transmit information regarding the energy availability in plants.