TEMPERATURE-SENSITIVE DNA SYNTHESIS IN A MUTANT OF BACILLUS SUBTILIS
TEMPERATURE-SENSITIVE DNA SYNTHESIS IN A MUTANT OF BACILLUS SUBTILIS
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DOI：
10.1099/00221287-56-1-87
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发表时间：
1969-01-01
期刊：
影响因子：
--
通讯作者：
RETIEF, Y
中科院分区：
文献类型：
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作者：
BAZILL, GW;RETIEF, Y
本文描述了枯草芽孢杆菌蛋白酶温度敏感突变株的性质，该突变株的DNA合成在40 ℃以上被特异性可逆地抑制。当在45°下孵育时，突变体中没有可检测到的DNA分解，并且脱氧核苷三磷酸的合成正常。温度敏感元件影响体内但不在体外的DNA合成，并且（与引发剂不同）它似乎是整个复制周期中DNA合成所必需的。
The properties of a temperature-sensitive mutant ofBacillus subtilisin which DNA synthesis is specifically and reversibly inhibited above 40° are described. When incubated at 45° there was no detectable breakdown of DNA in the mutant and the synthesis of deoxynucleoside triphosphates was normal. The temperature-sensitive element affects DNA synthesisin vivobut notin vitroand (unlike an initiator) it appears to be needed for DNA synthesis throughout the replication cycle.