MR imaging of a novel NOE-mediated magnetization transfer with water in rat brain at 9.4 T.
MR imaging of a novel NOE-mediated magnetization transfer with water in rat brain at 9.4 T.
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DOI：
10.1002/mrm.26396
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发表时间：
2017-08
影响因子：
3.3
通讯作者：
Zu Z
中科院分区：
文献类型：
--
作者：
Zhang XY;Wang F;Jin T;Xu J;Xie J;Gochberg DF;Gore JC;Zu Z
我们的目的是使用MRI在大鼠脑中检测，绘制和量化新型的核超肝增强（NOE）介导的磁化磁化；
优化了具有多种RF辐照能力的连续波MT序列，以达到9.4 T的最大对比度（-1.6）。还对重组模型磷脂以及两种细胞系（HEK293和9L）进行了MR测量，以研究该NOE的可能分子起源。
我们的结果表明，在大鼠大脑中可以可靠地检测到NOE（-1.6）的作用。与正常的脑组织中的测量相比，重新组装的磷脂的测量显着减少，这可能是胆碱磷脂的。
NOE（-1.6）可以用作检测脑肿瘤的新生物标志物。
We aimed to detect, map and quantify a novel nuclear Overhauser enhancement (NOE)-mediated magnetization transfer (MT) with water at around −1.6 ppm (NOE(−1.6)) in rat brain using MRI. Continuous wave MT sequences with a variety of RF irradiation powers were optimized to achieve the maximum contrast of this NOE(−1.6) effect at 9.4 T. The distribution of effect magnitudes, resonance frequency offsets, and line widths in healthy rat brains and the differences of the effect between tumors and contralateral normal brains were imaged and quantified using a multi-Lorentzian fitting method. MR measurements on reconstituted model phospholipids as well as two cell lines (HEK293 and 9L) were also performed to investigate the possible molecular origin of this NOE. Our results demonstrate that the NOE(−1.6) effect can be reliably detected in rat brain. Pixel-wise fittings demonstrate the regional variations of the effect. Measurements on rodent tumor model show that the amplitude of NOE(−1.6) in brain tumor is significantly diminished compared with that in normal brain tissue. Measurements on reconstituted phospholipids suggest that this effect may originate from choline phospholipids. The NOE(−1.6) could be used as a new biomarker for the detection of brain tumor.