Expression Cloning of Gamma Interferon-Inducing Antigens of Mycobacteriumavium subsp. paratuberculosis
Expression Cloning of Gamma Interferon-Inducing Antigens of Mycobacteriumavium subsp. paratuberculosis
复制标题
鸟分枝杆菌亚种γ干扰素诱导抗原的表达克隆。
作者：
R. Nagata;Y. Muneta;K. Yoshihara;Y. Yokomizo;Y. Mori
摘要/ ABSTRACT摘要：利用筛选得到的umavium亚种的核苷酸序列，制备了3个重组蛋白Map10、Map39和Map41。在大肠杆菌中表达的副结核菌基因组文库显著诱导受感染牛外周血单个核细胞产生γ干扰素。其中两个蛋白是PPE蛋白家族的成员。
ABSTRACT Three recombinant proteins, Map10, Map39, and Map41, produced based on nucleotide sequences obtained from the screening of Mycobacteriumavium subsp. paratuberculosis genomic library expressed in Escherichiacoli significantly elicited gamma interferon production in peripheral blood mononuclear cells from infected cattle. Two of these proteins were members of the PPE protein family.