The effect of recombinant interferon-alpha on lymphocyte subpopulations and HLA-DR expression on liver tissue of HBV-positive individuals.
The effect of recombinant interferon-alpha on lymphocyte subpopulations and HLA-DR expression on liver tissue of HBV-positive individuals.
复制标题
重组干扰素-α 对 HBV 阳性个体淋巴细胞亚群和肝组织 HLA-DR 表达的影响。
DOI：
10.1111/j.1365-2249.1990.tb05449.x
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发表时间：
1990
影响因子：
4.6
通讯作者：
vanThiel,DH
中科院分区：
文献类型：
--
作者：
Yoo,YK;Gavaler,JB;Chen,K;Whiteside,TL;vanThiel,DH
据报道，干扰素-α（IFN-α）在治疗由B型肝炎病毒（HBV）感染引起的慢性活动性肝炎中是有益的。已经提出用IFN-α治疗作为在临床肝移植中降低高的同种异体移植物感染率的手段，所述临床肝移植是针对B表面抗原（HBsAg）阳性的HBV相关慢性活动性肝炎和肝硬化而移植的患者。我们从两组接受肝移植的患者中获得了切除的全肝。A组为11例HBsAg阳性但未接受IFN-α治疗的患者，B组为10例HBsAg阳性但接受IFN-α治疗的患者，治疗时间为29.4 ± 5.6 d。两组之间在各种人口统计学和临床特征方面没有差异。通过抗生物素蛋白-生物素-免疫过氧化物酶技术，用针对不同单核细胞群体特有的细胞表面抗原的单克隆抗体对肝组织进行染色，以确定IFN-α对淋巴细胞亚群以及肝脏浸润单核细胞上HLA抗原表达的影响，肝内HLA-DR+淋巴细胞数量明显增多B组门脉区平均每高倍视野84 ± 14个病灶，A组33 ± 5个病灶，B组与A组比较差异有显著性（P< 0.005）。此外，B组汇管区（P< 0.02）和肝小叶（P < 0.05）淋巴细胞HLA-DR抗原表达强度高于A组。B组汇管区NK细胞数量较A组明显增加（P< 0.05）。肝脏中存在的淋巴细胞和NK细胞群对IFN-α治疗的反应的这些变化可能反映了IFN-α诱导的对病毒感染细胞的免疫应答增强。
Interferon-alpha (IFN-α) has been reported to be beneficial in the treatment of chronic active hepatitis occurring as a result of hepatitis B virus (HBV) infection. Treatment with IFN-a has been proposed as a means of reducing the high rate of allograft infection in clinical liver transplantation in patients transplanted for HBV-related chronic active hepatitis and cirrhosis who are positive for hepatitis B surface antigen (HBsAg). We obtained resected whole livers from two groups of patients who received liver transplants. Group A consisted of 11 patients who were HBsAg+but were not treated with IFN-α, and group B consisted of 10 patients who were also HBsAg+but received IFN-α therapy for 29.4 ± 5.6 days prior to orthotopic liver transplantation. No differences between the two groups existed in terms of a variety of demographic and clinical characteristics. The liver tissue was stained with monoclonal antibodies to cell surface antigens unique to different mononuclear cell populations by the avidin-biotin-immunoperoxidase technique to determine the effect of IFN-α on the lymphocyte subsets as well as HLA antigen expression on liver-infiltrating mononuclear cells, The number of HLA-DR+lymphocytes in the liver was significantly increased (P< 0.005) within the portal areas in group B compared with that found in group A (84 ± 14versus33 ± 5 per one high-power field). Moreover, the intensity of the HLA-DR antigen expression on lymphocytes in the portal areas (P< 0.02) and in the hepatic lobule (P < 0.05) was greater in group B than in group A. The number of natural killer (NK) cells was increased in the portal areas (P< 0.05) of group B compared with group A. These alterations in the lymphocyte and NK cell populations present in the liver in response to IFN-α therapy presumably reflect an IFN-α-induced enhancement of the immune response to virus-infected cells.