In situ measurement of the stiffness increase in the posterior sclera after UV-riboflavin crosslinking by optical coherence elastography.
In situ measurement of the stiffness increase in the posterior sclera after UV-riboflavin crosslinking by optical coherence elastography.
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DOI：
10.1364/boe.463600
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发表时间：
2022-08
影响因子：
3.4
通讯作者：
Maria Vinas-Pena;Xu Feng;Guo-Yang Li;S. Yun
中科院分区：
文献类型：
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作者：
Maria Vinas-Pena;Xu Feng;Guo-Yang Li;S. Yun
巩膜交联可以提供一种通过硬化巩膜组织来预防或治疗近视的方法。在巩膜交联前后原位测量巩膜组织硬度的能力将是有用的，但尚未建立。在这里，我们测试了光学相干弹性成像（OCE）测量巩膜组织的剪切模量的可行性，并使用离体猪眼作为模型来评估交联对不同后巩膜区域的影响。从测量的弹性波速度在6 - 16 kHz，我们得到的平面外剪切模量值为0.71 ± 0.12 MPa（n = 20）的正常猪巩膜组织。在核黄素辅助的UV交联后，剪切模量增加到1.50 ± 0.39MPa（n = 20）。这种2倍的变化与交联后静态杨氏模量从5.5 ± 1.1 MPa增加到9.3 ± 1.9 MPa一致，这是我们使用常规单轴拉伸法在组织条上测量的。OCE显示颞部、鼻部和深层后巩膜的区域硬度差异。我们的研究结果表明，OCE作为一种非侵入性的工具，以评估巩膜交联的效果的潜力。
Scleral crosslinking may provide a way to prevent or treat myopia by stiffening scleral tissues. The ability to measure the stiffness of scleral tissues in situ pre and post scleral crosslinking would be useful but has not been established. Here, we tested the feasibility of optical coherence elastography (OCE) to measure shear modulus of scleral tissues and evaluate the impact of crosslinking on different posterior scleral regions using ex vivo porcine eyes as a model. From measured elastic wave speeds at 6 - 16 kHz, we obtained out-of-plane shear modulus value of 0.71 ± 0.12 MPa (n = 20) for normal porcine scleral tissues. After riboflavin-assisted UV crosslinking, the shear modulus increased to 1.50 ± 0.39 MPa (n = 20). This 2-fold change was consistent with the increase of static Young's modulus from 5.5 ± 1.1 MPa to 9.3 ± 1.9 MPa after crosslinking, which we measured using conventional uniaxial extensometry on tissue stripes. OCE revealed regional stiffness differences across the temporal, nasal, and deeper posterior sclera. Our results show the potential of OCE as a noninvasive tool to evaluate the effect of scleral crosslinking.