CIRCADIAN CLOCK MUTANTS OF CYANOBACTERIA
CIRCADIAN CLOCK MUTANTS OF CYANOBACTERIA
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DOI：
10.1126/science.7973706
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发表时间：
1994-11-18
期刊：
影响因子：
56.9
通讯作者：
ISHIURA, M
中科院分区：
文献类型：
--
作者：
KONDO, T;TSINOREMAS, NF;ISHIURA, M
一组不同的生物钟突变体分离的蓝藻菌株，携带细菌荧光素酶报告基因连接到一个时钟控制的启动子。在150，000个化学诱变的生物发光细胞克隆中，分离出12个突变体，它们表现出广谱的周期（16至60小时），发现5个突变体表现出各种不寻常的模式，包括心律失常。这些突变似乎是时钟特异性的。此外，它被证明，在这种蓝藻是可能的，通过互补克隆突变基因，这提供了一种手段，从遗传上剖析昼夜节律机制。
A diverse set of circadian clock mutants was isolated in a cyanobacterial strain that carries a bacterial luciferase reporter gene attached to a clock-controlled promoter. Among 150,000 clones of chemically mutagenized bioluminescent cells, 12 mutants were isolated that exhibit a broad spectrum of periods (between 16 and 60 hours), and 5 mutants were found that show a variety of unusual patterns, including arrhythmia. These mutations appear to be clock-specific. Moreover, it was demonstrated that in this cyanobacterium it is possible to clone mutant genes by complementation, which provides a means to genetically dissect the circadian mechanism.