One-step synthesis of thermally stable artificial multienzyme cascade system for efficient enzymatic electrochemical detection
One-step synthesis of thermally stable artificial multienzyme cascade system for efficient enzymatic electrochemical detection
复制标题
一步合成热稳定人工多酶级联系统用于高效酶电化学检测
DOI：
10.1007/s12274-019-2548-8
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发表时间：
2019-11-06
期刊：
影响因子：
9.9
通讯作者：
Zheng, Lansun
中科院分区：
文献类型：
--
作者：
Cheng, Xiqing;Zhou, Jinhong;Zheng, Lansun
近年来，基于金属-有机框架（MOF）的多酶系统由于具有较高的催化效率和在传感领域的应用前景而受到越来越多的关注。酶或纳米酶在mof内的简单可控整合是实现高效级联催化和高稳定性的关键。在这里，我们报告了一种简单的电化学辅助仿生矿化策略，以制备一种人工多酶系统，用于高效的生物分子电化学检测。利用GOx@Cu-MOF/泡沫铜（GOx@Cu-MOF/CF）结构作为概念证明，通过简单的一步电化学辅助仿生矿化策略，将葡萄糖氧化酶（GOx）原位包封在三维（3D）多孔导电CF上生长的mof层中，实现了高效的酶固定化和级联催化。由于生物电催化级联反应机制，这种精心设计的GOx@Cu-MOF修饰电极在葡萄糖传感方面表现出优异的催化活性和热稳定性。值得注意的是，GOx@Cu-MOF/CF在80℃孵育后活性仍保持在约80%。与此形成鲜明对比的是，未经保护的电极经过同样的处理后，活性降低到原来的10%。本文提出的设计策略可用于制备高稳定性的enzyme@MOF复合材料，应用于高效光热治疗和其他高温平台。
Recently, metal-organic framework (MOF)-based multienzyme systems integrating different functional natural enzymes and/or nanomaterial-based artificial enzymes are attracting increasing attention due to their high catalytic efficiency and promising application in sensing. Simple and controllable integration of enzymes or nanozymes within MOFs is crucial for achieving efficient cascade catalysis and high stability. Here, we report a facile electrochemical assisted biomimetic mineralization strategy to prepare an artificial multienzyme system for efficient electrochemical detection of biomolecules. By using the GOx@Cu-MOF/copper foam (GOx@Cu-MOF/CF) architecture as a proof of concept, efficient enzyme immobilization and cascade catalysis were achieved byin situencapsulation of glucose oxidase (GOx) within MOFs layer grown on three-dimensional (3D) porous conducting CF via a facile one-step electrochemical assisted biomimetic mineralization strategy. Due to the bio-electrocatalytic cascade reaction mechanism, this well-designed GOx@Cu-MOF modified electrode exhibited superior catalytic activity and thermal stability for glucose sensing. Notably, the activity of GOx@Cu-MOF/CF still remained at ca. 80% after being incubated at 80 °C. In sharp contrast, the activity of the unprotected electrode was reduced to the original 10% after the same treatment. The design strategy presented here may be useful in fabricating highly stable enzyme@MOF composites applied for efficient photothermal therapy and other platform under high temperature.