Deviated mechanism of wound healing in diabetic corneas
Deviated mechanism of wound healing in diabetic corneas
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DOI：
10.1097/ico.0b013e31812f6d8e
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发表时间：
2007-10-01
期刊：
影响因子：
2.8
通讯作者：
Nishida, Teruo
中科院分区：
文献类型：
--
作者：
Chikama, Tai-ichiro;Wakuta, Makiko;Nishida, Teruo
我们研究了2型糖尿病自发性模型Goto-Kakizaki （GK）大鼠角膜上皮伤口愈合过程中的表型变化。在本文中，我们概述了我们和其他小组的研究，并描述了糖尿病角膜病变的临床特征。我们观察到，与Wistar大鼠相比，GK大鼠角膜上皮伤口愈合率降低。在GK大鼠角膜上皮基底膜附近的2层细胞中检测到Cx43、k14和Ki-67蛋白的免疫反应性，而Wistar大鼠角膜上皮中只有单个基底层细胞对这些蛋白呈阳性反应。在完整角膜上皮和创面愈合过程中，GK大鼠ki -67阳性细胞的频率高于Wistar大鼠。GK大鼠表现出延迟的角膜上皮伤口愈合以及在人类糖尿病性角膜病变中观察到的情况。此外，这些结果表明糖尿病患者角膜上皮细胞功能偏差的可能性。
We examined phenotypic changes during the wound healing process in the corneal epithelium of Goto-Kakizaki (GK) rats, a spontaneous model of type 2 diabetes mellitus. In this article, we provide an overview of our and other groups' research and describe the clinical features of diabetic keratopathy. We observed that the rate of corneal epithelial wound closure was decreased in GK rats compared with Wistar rats. Immunoreactivity for Cx43, K 14, and Ki-67 was detected in the 2 layers of cells adjacent to the basement membrane in the corneal epithelium of GK rats, whereas only the single basal layer of cells was positive for these proteins in the corneal epithelium of Wistar rats. The frequency of Ki-67-positive cells was greater in GK rats than in Wistar rats in the intact corneal epithelium and during wound healing. The GK rat represents delayed corneal epithelial wound closure as well as that which is observed in human diabetic keratopathy. Furthermore, these results indicate a possibility of functional deviation in corneal epithelial cells with diabetes mellitus.