TRANSGENIC ROOT-NODULES OF VICIA-HIRSUTA - A FAST AND EFFICIENT SYSTEM FOR THE STUDY OF GENE-EXPRESSION IN INDETERMINATE-TYPE NODULES
TRANSGENIC ROOT-NODULES OF VICIA-HIRSUTA - A FAST AND EFFICIENT SYSTEM FOR THE STUDY OF GENE-EXPRESSION IN INDETERMINATE-TYPE NODULES
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DOI：
10.1094/mpmi-6-699
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发表时间：
1993-11-01
影响因子：
3.5
通讯作者：
BROER, I
中科院分区：
文献类型：
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作者：
QUANDT, HJ;PUHLER, A;BROER, I
发根农杆菌介导的毛野豌豆转化导致在幼苗受伤的上胚轴上诱导转基因“毛状根”。一旦“毛状根”发育得足够好，原始野生型根就被切除，从而建立了由未转化芽上的转基因根组成的复合植物。通过β-葡萄糖醛酸酶（GUS）表达证明转基因的根比例达到88%。用豆根瘤菌 bv 接种复合植物。在 V. hirsuta 幼苗被 A. rhizogenes 感染后不到 20 天，viciae 就导致了转基因不确定型根瘤的诱导。转基因根瘤的结构和功能与野生型根瘤相当。为了说明该系统的适用性，在根和根瘤发育过程中监测了包含内含子的 p35S-gusA-int 基因融合体的空间和时间表达模式。
The Agrobacterium rhizogenes-mediated transformation of Vicia hirsuta resulted in the induction of transgenic 'hairy roots' on wounded epicotyls of young seedlings. As soon as the 'hairy roots' had developed enough, the original wild-type roots were excised and composite plants were thus established that consisted of transgenic roots on untransformed shoots. The proportion of roots that proved by their beta-glucuronidase (GUS) expression to be transgenic reached 88%. The inoculation of composite plants with Rhizobium leguminosarum bv. viciae led to the induction of transgenic indeterminate-type root nodules in less than 20 days after the V. hirsuta seedlings had been infected with A. rhizogenes. The structure and the function of the transgenic nodules were comparable to that of wildtype nodules. To illustrate the applicability of the system, the spatial and temporal expression pattern of an intron-containing p35S-gusA-int gene fusion was monitored during root and nodule development.