THE ENHANCER OF SPLIT [E(SPL)] LOCUS OF DROSOPHILA ENCODES 7 INDEPENDENT HELIX LOOP HELIX PROTEINS
THE ENHANCER OF SPLIT [E(SPL)] LOCUS OF DROSOPHILA ENCODES 7 INDEPENDENT HELIX LOOP HELIX PROTEINS
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DOI：
10.1073/pnas.89.18.8731
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发表时间：
1992-09-15
影响因子：
11.1
通讯作者：
ARTAVANISTSAKONAS, S
中科院分区：
文献类型：
--
作者：
DELIDAKIS, C;ARTAVANISTSAKONAS, S
E（spl）基因座被认为参与了细胞相互作用机制，该机制控制果蝇发育过程中许多细胞命运的选择，包括神经前体的分离。先前的研究表明，E（spl）由两组密切相关的转录物定义，（i）编码具有螺旋-环-螺旋（HLH）基序的蛋白质的三个转录物的簇和（ii）编码含有重复基序的核蛋白的单拷贝基因，所述重复基序首先在鸟嘌呤核苷酸结合蛋白的β亚基中鉴定。这两个群体在基因上都与编码跨膜蛋白的Notch基因座相互作用。我们报告了四个额外的HLH编码基因的结构，这些基因位于E（spl）复合体中，并提供了证据，证明我们现在已经确定了E（spl）HLH簇的所有剩余成员。
The E(spl) locus is thought to participate in a cell interaction mechanism that controls the choice of many cell fates during Drosophila development, including the segregation of neural precursors. Previous studies have demonstrated that E(spl) is defined by two groups of closely related transcripts, (i) a cluster of three transcripts encoding proteins bearing a helix-loop-helix (HLH) motif and (ii) a single-copy gene encoding a nuclear protein containing repeated motifs first identified in the beta-subunit of guanine nucleotide-binding proteins. Both groups interact genetically with the Notch locus, which codes for a transmembrane protein. We report the structure of four additional HLH-encoding genes that reside in the E(spl) complex and provide evidence that we have now identified all the remaining members of the E(spl) HLH cluster.