In vivo technique for determining transcriptional mutagenesis.
In vivo technique for determining transcriptional mutagenesis.
复制标题
用于确定转录突变的体内技术。
DOI：
10.1006/meth.2000.1052
复制
发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Doetsch,PW
中科院分区：
文献类型：
--
作者：
You,HJ;Viswanathan,A;Doetsch,PW
When an elongating RNA polymerase encounters DNA damage on the template strand of a transcribed gene it can either be arrested by or be transcribed through the lesion. Lesions that arrest RNA polymerases are thought to be subject to transcription-coupled repair, whereas that damage that is bypassed can cause miscoding, resulting in “mutations” in the transcript (transcriptional mutagenesis). We have developed a technique using a plasmid-based luciferase reporter assay to determine the extent to which a particular type of DNA base modification is capable of causing transcriptional mutagenesis in vivo. The system uses Escherichia coli strains with different DNA repair backgrounds and is designed to detect phenotypic changes caused by transcriptional mutagenesis under nongrowth conditions. In addition, this method is capable of indicating the extent to which a particular DNA repair enzyme (or pathway) suppresses the occurrence of transcriptional mutagenesis. Thus, this technique provides a tool with which the effects of various genes on non-replication-dependent pathways resulting in the generation of mutant proteins can be gauged.
逐步去除萤火虫荧光素酶 C 端 12 个氨基酸会导致活性逐渐丧失。
DOI：
10.1016/0167-4838(94)90084-1
发表时间：
1994
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
G. Sala;A. K. Campbell
通讯作者：
A. K. Campbell
生物发光和化学发光。由 Philip E. Stanley 和 Larry J. Kricka 编辑，第 570 页。Wiley，Chichester，1991 年。80.00 英镑。
影响因子：
0.6
作者：
J. Lancaster
通讯作者：
J. Lancaster
胰蛋白酶抑制剂反式 4-胍基甲基环己烷甲酸 4-叔丁基苯酯可抑制大肠杆菌细胞中因磷酸盐饥饿而同步的 DNA 合成的开始。
影响因子：
2
作者：
M. Katô;T. Irisawa;Y. Morimoto;M. Muramatu
通讯作者：
M. Muramatu