Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter
Enhanced heat and drought tolerance in transgenic rice seedlings overexpressing OsWRKY11 under the control of HSP101 promoter
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DOI：
10.1007/s00299-008-0614-x
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发表时间：
2008
影响因子：
6.2
通讯作者：
Xiaolan Wu;Yoko Shiroto;S. Kishitani;Yukihiro Ito;K. Toriyama
中科院分区：
文献类型：
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作者：
Xiaolan Wu;Yoko Shiroto;S. Kishitani;Yukihiro Ito;K. Toriyama
AnOsWRKY11基因编码一个带有WRKY结构域的转录因子，是水稻幼苗在热激和干旱胁迫下共同诱导的基因之一。为了确定OsWRKY11基因的过表达是否具有耐热性和抗旱性，将OsWRKY11基因与水稻HSP101启动子融合，并导入水稻品种Sasanishiki。热处理诱导了OsWRKY11的过表达。经过热预处理后，转基因株系表现出显著的耐热性和抗旱性，表现为叶片萎靡较慢，植株绿色部分的存活率较低。它们还表现出显著的耐干性，表现为离体叶的失水速度较慢。我们的结果表明，OsWRKY11基因在高温和干旱胁迫反应和耐性中发挥作用，可能有助于提高抗逆性。
AnOsWRKY11gene, which encodes a transcription factor with the WRKY domain, was identified as one of the genes that was induced by both heat shock and drought stresses in seedlings of rice (Oryza sativaL.). To determine if overexpression ofOsWRKY11confers heat and drought tolerance,OsWRKY11cDNA was fused to the promoter ofHSP101of rice and introduced into a rice cultivar Sasanishiki. Overexpression ofOsWRKY11was induced by heat treatment. After heat pretreatment, the transgenic lines showed significant heat and drought tolerance, as indicated by the slower leaf-wilting and less-impaired survival rate of green parts of plants. They also showed significant desiccation tolerance, as indicated by the slower water loss in detached leaves. Our results indicate that theOsWRKY11gene plays a role in heat and drought stress response and tolerance, and might be useful for improvement of stress tolerance.