NK-cells have an impaired response to acute exercise and a lower expression of the inhibitory receptors KLRG1 and CD158a in humans with latent cytomegalovirus infection
NK-cells have an impaired response to acute exercise and a lower expression of the inhibitory receptors KLRG1 and CD158a in humans with latent cytomegalovirus infection
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DOI：
10.1016/j.bbi.2011.09.004
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发表时间：
2012-01-01
影响因子：
15.1
通讯作者：
Simpson, Richard J.
中科院分区：
文献类型：
--
作者：
Bigley, Austin B.;Lowder, Thomas W.;Simpson, Richard J.
NM细胞和γ δ T细胞是免疫系统的细胞毒性效应物，其响应于急性应激和运动而优先动员到血液隔室中。虽然已知感染史会改变CD 8 + T细胞的表型和运动反应性，但潜伏性巨细胞病毒（CMV）和EB病毒（EBV）感染对NM细胞和γ δ T细胞的表型和运动反应性的影响尚不清楚。20名健康男性（年龄：28.4 +/- 5.4岁）在85%峰值功率下循环30分钟。通过四色流式细胞术对运动前、运动后即刻和运动后1 h分离的血液淋巴细胞进行CD 3、CD 4、CD 8、CD 56、CD 57、CD 158 a、KLRG 1和γ δ-TCR抗原的表面染色。通过ELISA测定CMV和EBV血清状态（阳性/阴性）。与CMV阴性相比，CMVpos具有较低比例的表达抑制性受体（KLRG 1+和CD 158 a+）的NM细胞和较高比例的终末分化NM细胞（KLRG 1-/CD 57+）。尽管与CMV阴性相比具有相似的基线NM细胞计数和对运动的生理反应，但CMVpos对运动的动员少得多（132个细胞/μ L对245个细胞/μ L），尽管终末分化的NM细胞对运动的反应与CMV血清状态无关（p = 0.658）。EBVpos具有较高比例的CD 8 + NM细胞，但细胞对运动的反应不受EBV的影响。γ δ T细胞的频率和运动反应性不受CMV或EBV血清状态的影响（p > 0.05）。总之，潜伏性CMV感染与表达抑制性受体的NM细胞的数量减少和NM细胞对急性运动的响应的钝化动员有关。这可能表明在那些感染CMV的人中对“战斗或逃跑”情况的免疫应答受损。(C)2011 Elsevier Inc. All rights reserved.
NM-cells and gamma delta T-cells are cytotoxic effectors of the immune system that are preferentially mobilized into the blood compartment in response to acute stress and exercise. While infection history is known to alter the phenotype and exercise-responsiveness of CD8+ T-cells, the influence of latent cytomegalovirus (CMV) and Epstein-Barr virus (EBV) infections on the phenotypes and exercise-responsiveness of NM-cells and gamma delta T-cells are unknown. Twenty healthy males (age: 28.4 +/- 5.4 years) cycled for 30 min at 85% peak power. Blood lymphocytes isolated before, immediately after, and 1 h after exercise were surface stained for CD3, CD4, CD8, CD56, CD57, CD158a, KLRG1, and gamma delta-TCR antigens by four-color flow cytometry. CMV and EBV serostatus (pos/neg) was determined by ELISA. CMVpos had lower proportions of NM-cells expressing inhibitory receptors (KLRG1+ and CD158a+) and higher proportions of terminally differentiated NM-cells (KLRG1-/CD57+) compared to CMVneg. CMVpos mobilized far fewer (132 cells/mu L vs. 245 cells/mu L) NM-cells in response to exercise despite having similar baseline NM-cell counts and physiological responses to exercise as CMVneg, although terminally differentiated NM-cells were equally responsive to exercise regardless of CMV serostatus (p = 0.658). EBVpos had higher proportions of CD8+ NM-cells, but cellular responses to exercise were not influenced by EBV. The frequency and exercise-responsiveness of gamma delta T-cells was not affected by CMV or EBV serostatus (p > 0.05). In conclusion, latent CMV infection is associated with lowered numbers of NM-cells expressing inhibitory receptors and a blunted mobilization of NM-cells in response to acute exercise. This may indicate a compromised immune response to "fight-or-flight" situations in those infected with CMV. (C) 2011 Elsevier Inc. All rights reserved.