Faecal microbiota composition and host-microbe cross-talk following gastroenteritis and in postinfectious irritable bowel syndrome
Faecal microbiota composition and host-microbe cross-talk following gastroenteritis and in postinfectious irritable bowel syndrome
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DOI：
10.1136/gutjnl-2013-305994
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发表时间：
2014-11-01
期刊：
影响因子：
24.5
通讯作者：
de Vos, Willem M.
中科院分区：
文献类型：
--
作者：
Jalanka-Tuovinen, Jonna;Salojarvi, Jarkko;de Vos, Willem M.
大约10%的IBS患者报告在感染性肠炎后开始出现综合征。感染后IBS（PI-IBS）的临床特征类似于大肠杆菌为主的IBS（IBS-D）。虽然已经在其他IBS亚型中鉴定了改变的粪便微生物群，但是PI-IBS患者中的微生物群的组成仍然未被表征。目的鉴定PI-IBS患者的微生物组成，并且检查粪便微生物群与患者的临床特征之间的关联。设计使用系统发育微阵列和选择的qPCR测定，我们分析了来自五个研究组的57名受试者的粪便微生物群的差异：诊断为PI-IBS的患者，胃肠炎后6个月具有持续性肠功能障碍或无IBS症状的患者，以IBS-D患者和健康对照为基准。此外，粪便微生物群和健康之间的关联进行了研究，通过相关的微生物档案免疫标记，生活质量指标和宿主基因表达在直肠biopsies.Results微生物群分析显示了27个属样组的细菌配置文件，提供了一个指数的微生物菌群失调（IMD），显着分离患者组和对照组。在这种情况下，拟杆菌门的几个成员在患者中增加了12倍，而健康对照组的未培养梭菌则增加了35倍。我们发现IMD和几个宿主基因途径，包括氨基酸合成，细胞连接的完整性和炎症反应的表达之间的相关性，这表明在IBS的上皮屏障功能受损。结论与PI-IBS患者的粪便微生物群不同于健康对照组，类似于IBS-D患者，这表明一个共同的病理生理。此外，我们的分析表明，各种宿主微生物协会可能是肠道症状的基础，由胃肠炎发起。
Background About 10% of patients with IBS report the start of the syndrome after infectious enteritis. The clinical features of postinfectious IBS (PI-IBS) resemble those of diarrhoea-predominant IBS (IBS-D). While altered faecal microbiota has been identified in other IBS subtypes, composition of the microbiota in patients with PI-IBS remains uncharacterised.Objective To characterise the microbial composition of patients with PI-IBS, and to examine the associations between the faecal microbiota and a patient's clinical features.Design Using a phylogenetic microarray and selected qPCR assays, we analysed differences in the faecal microbiota of 57 subjects from five study groups: patients with diagnosed PI-IBS, patients who 6 months after gastroenteritis had either persisting bowel dysfunction or no IBS symptoms, benchmarked against patients with IBS-D and healthy controls. In addition, the associations between the faecal microbiota and health were investigated by correlating the microbial profiles to immunological markers, quality of life indicators and host gene expression in rectal biopsies.Results Microbiota analysis revealed a bacterial profile of 27 genus-like groups, providing an Index of Microbial Dysbiosis (IMD), which significantly separated patient groups and controls. Within this profile, several members of Bacteroidetes phylum were increased 12-fold in patients, while healthy controls had 35-fold more uncultured Clostridia. We showed correlations between the IMD and expression of several host gene pathways, including amino acid synthesis, cell junction integrity and inflammatory response, suggesting an impaired epithelial barrier function in IBS.Conclusions The faecal microbiota of patients with PI-IBS differs from that of healthy controls and resembles that of patients with IBS-D, suggesting a common pathophysiology. Moreover, our analysis suggests a variety of host-microbe associations that may underlie intestinal symptoms, initiated by gastroenteritis.