ISOLATION OF FACTOR-XA FROM CHICK-EMBRYO AS THE AMNIOTIC ENDOPROTEASE RESPONSIBLE FOR PARAMYXOVIRUS ACTIVATION
ISOLATION OF FACTOR-XA FROM CHICK-EMBRYO AS THE AMNIOTIC ENDOPROTEASE RESPONSIBLE FOR PARAMYXOVIRUS ACTIVATION
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DOI：
10.1016/0014-5793(92)80303-x
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发表时间：
1992-01-27
期刊：
影响因子：
3.5
通讯作者：
NAGAI, Y
中科院分区：
文献类型：
--
作者：
GOTOH, B;YAMAUCHI, F;NAGAI, Y
在鸡胚中，某些副粘病毒主要以绒毛膜尿囊和尿囊羊膜为感染目标，在其他器官中没有进一步的广泛传播。这可以解释为在尿囊液和羊水中可能存在激活病毒融合糖蛋白前体的内源性蛋白酶，而在其他部位或器官中不存在。我们以前从尿囊液中分离出这种内源性蛋白酶，并证明了它与Xa凝血因子的同一性。通过包括n端氨基酸序列在内的所有标准，从羊水中分离出完全相同的内源性蛋白酶。因此，Xa因子似乎是鸡胚病毒嗜性的主要宿主决定因素。
In chick embryo, certain paramyxoviruses mainly target the chorioallantois and the allantoamnion and show no extensive further spreading in the other organs. This has been explained by the possible presence of an endoprotease activating the viral fusion glycoprotein precursor in the allantoic and the amniotic fluid, and its absence in other places or organs. We previously isolated such an endoprotease from the allantoic fluid and demonstrated its identity with the clotting factor Xa. Exactly the same endoprotease by all the criteria including the N-terminal amino acid sequence was now isolated from the amniotic fluid. Thus, the factor Xa seems to be a major host determinant of the viral tropism in chick embryo.