Novel registration for microcomputed tomography and bioluminescence imaging based on iterated optimal projection
Novel registration for microcomputed tomography and bioluminescence imaging based on iterated optimal projection
复制标题
基于迭代最优投影的微计算机断层扫描和生物发光成像的新型配准
DOI：
10.1117/1.jbo.18.2.026013
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发表时间：
2013
影响因子：
3.5
通讯作者：
Tian Jie
中科院分区：
文献类型：
--
作者：
Ma Xibo;Deng Kexin;Xue Zhenwen;Liu Xueyan;Zhu Shouping;Qin Chenghu;Yang Xin;Tian Jie
抽象。作为一种高灵敏度的成像方式，生物发光断层成像可以基于三维表面光分布重建内部发光源的三维（3-D）位置。然而，在实验中，我们只能得到多方位的二维（2-D）生物发光分布。因此，发展一种精确的通用配准方法对于后续的生物光源重建至关重要。我们提出了一种基于迭代最优投影的2-D到3-D配准方法，并将其应用于3只转基因小鼠的配准和重建研究。与传统的基于固定点的配准方法相比，该方法与标记点无关，三个实验的配准精度分别提高了0.3、0.5和0.4个像素。此外，基于上述两种配准方法的配准结果，我们重建了三只转基因小鼠体内生物发光源的三维位置。重建结果表明，重建单元中心与真实的单元中心的平均误差距离分别减小了0.32、0.48和0.39 mm。
Abstract. As a high-sensitivity imaging modality, bioluminescence tomography can reconstruct the three-dimensional (3-D) location of an internal luminescent source based on the 3-D surface light distribution. However, we can only get the multi-orientation two-dimensional (2-D) bioluminescence distribution in the experiments. Therefore, developing an accurate universal registration method is essential for following bioluminescent source reconstruction. We can then map the multi-orientation 2-D bioluminescence distribution to the 3-D surface derived from anatomical information with it. We propose a 2-D -to-3-D registration method based on iterated optimal projection and applied it in a registration and reconstruction study of three transgenic mice. Compared with traditional registration methods based on the fixed points, our method was independent of the markers and the registration accuracy of the three experiments was improved by 0.3, 0.5, and 0.4 pixels, respectively. In addition, based on the above two registration results using the two registration methods, we reconstructed the 3-D location of the inner bioluminescent source in the three transgenic mice. The reconstruction results showed that the average error distance between the center of the reconstructed element and the center of the real element were reduced by 0.32, 0.48, and 0.39 mm, respectively.