Involvement of Ypt7p, a small GTPase, in traffic from late endosome to the vacuole in yeast.
Involvement of Ypt7p, a small GTPase, in traffic from late endosome to the vacuole in yeast.
复制标题
DOI：
--
复制
发表时间：
1993-11
影响因子：
4
通讯作者：
F. Schimmöller;H. Riezman
中科院分区：
文献类型：
--
作者：
F. Schimmöller;H. Riezman
YPT 7基因编码哺乳动物rab 7蛋白的酿酒酵母同源物。从对Δ ypt 7突变体的研究中获得的数据表明，Ypt 7 p参与酵母中的内吞途径（Wichmann等人，Cell 71，1131-1142，1992）。我们在这里报告，内吞信息素α-因子积累在晚期内体在δ ypt 7细胞，表明Ypt 7 p参与调节运输步骤从晚期内体的液泡。我们还表明，α-因子可以降解的PEP-4依赖的方式在一个prevacuolar/内体隔室在δ ypt 7细胞，提供独立的证据表明，空泡生物发生和内吞在酵母中的途径可能相交的内体膜系统。
The YPT7 gene encodes the Saccharomyces cerevisiae homolog of mammalian rab7 protein. Data obtained from studies on a delta ypt7 mutant suggested that Ypt7p is involved in the endocytic pathway in yeast (Wichmann et al., Cell 71, 1131-1142, 1992). We report here that endocytosed pheromone alpha-factor accumulates in late endosomes in delta ypt7 cells, indicating that Ypt7p is involved in the regulation of transport steps from late endosomes to the vacuole. We also show that alpha-factor can be degraded in a PEP4-dependent manner in a prevacuolar/endosomal compartment in delta ypt7 cells, providing independent evidence that the pathways of vacuole biogenesis and endocytosis in yeast may intersect in the endosomal membrane system.