The effect of endogenous nitric oxide on cholinergic ciliary stimulation of human nasal mucosa
The effect of endogenous nitric oxide on cholinergic ciliary stimulation of human nasal mucosa
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DOI：
10.1097/00005537-200409000-00026
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发表时间：
2004-09-01
期刊：
影响因子：
2.6
通讯作者：
Rudack, C
中科院分区：
文献类型：
--
作者：
Alberty, J;August, C;Rudack, C
目标/假设。变应性鼻炎患者鼻纤毛呼吸道上皮中诱导型一氧化氮合酶产生的内源性一氧化氮（NO）增加。最近的实验数据表明，内源性NO强烈参与纤毛活动的复杂调节，粘液纤毛运输系统的驱动力。作者研究了内源性NO对乙酰胆碱刺激的人鼻粘膜纤毛活动的影响。研究设计：体外研究。方法.培养人鼻粘膜外植体与肿瘤坏死因子-a和细菌脂多糖孵育，以提高内源性NO的产生。诱导型NO合酶的表达通过免疫组织化学进行形态学证实。纤毛搏动频率通过纤毛上皮的相差显微镜，使用计算机光电技术确定。用乙酰胆碱和NO合成酶抑制剂N（G）-硝基-L-精氨酸甲酯（L-NAME）进行体外刺激实验。结果通过免疫组化分析，观察了用肿瘤坏死因子-a和脂多糖刺激后呼吸道上皮中诱导型NO合酶的上调。10（-2）mol/L L-NAME抑制内源性NO的产生可显著降低纤毛基础频率（8.6 ± 0.2 Hz）至7.8 ± 0.2 Hz（P
Objectives/Hypothesis. Endogenous nitric oxide (NO) production by inducible nitric oxide synthase is enhanced in the nasal ciliated respiratory tract epithelium of patients with allergic rhinitis. Recent experimental data have suggested endogenous NO to be strongly involved in the complex regulation of ciliary activity, the driving force of the mucociliary transport system. The authors investigated the effect of endogenous NO on acetylcholine-stimulated ciliary activity of human nasal mucosa. Study Design: In vitro study. Methods. Cultures of human nasal mucosa explants were incubated with tumor necrosis factor-a and bacterial lipopolysaccharides to enhance endogenous NO production. Expression of inducible NO synthase was morphologically demonstrated by immunohistochemistry. Ciliary beat frequency was determined by phase-contrast microscopy of ciliated epithelium, using a computerized photoelectric technique. Stimulation experiments were performed in vitro with acetylcholine and N(G)-nitro-l-arginine methyl ester (L-NAME), a NO synthase inhibitor. Results. Upregulation of inducible NO synthase in the respiratory tract epithelium after stimulation with tumor necrosis factor-a and lipopolysaccharide was visualized by immunohistochemical analysis. Experimental inhibition of enhanced endogenous NO production by 10(-2) mol/L L-NAME significantly reduced baseline ciliary beat frequency from 8.6 +/- 0.2 to 7.8 +/- 0.2 Hz (P