Correlation between whitefly (Homoptera: Aleyrodidae) feeding behavior and transmission of tomato yellow leaf curl virus
Correlation between whitefly (Homoptera: Aleyrodidae) feeding behavior and transmission of tomato yellow leaf curl virus
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DOI：
10.1603/0013-8746(2000)093
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发表时间：
2000-05-01
影响因子：
2.3
通讯作者：
Fereres, A
中科院分区：
文献类型：
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作者：
Jiang, YX;De Blas, C;Fereres, A
利用电子穿透图技术（EPG）对番茄黄化曲叶双生病毒（TYLCV）传播过程中烟粉虱的取食行为进行了监测。在两叶期番茄试验植物（Lycopersicon esculentum Mill 'Riofuego'）上记录个体带病毒粉虱的行为。在3.5至14 h的接种访问期间，在单个测试植物上记录了总共213个粉虱个体。根据所观察到的波形将记录分为4类：I组，仅包括波形C（pathway），TYLCV的剩余传输效率为2.4%，II组，显示探针路径和单个E（pd）（1）波形，传输效率为7.4%; Ⅲ组为单波E（pd）（1）+ E（pd）（2），为针径传播，传播效率为23.4%;最后是TV组，显示了多个E（pd）（1）+ E（pd）（2）波形的路径，与最高传输效率（37.5%）相关。共16个不同的行为变量被引入到逐步向后逻辑回归模型，以确定与TYLCV接种最相关的变量。其中，E（pd）（1）的总持续时间是与B接种病毒相关性最显著的变量。烟粉虱（P = 0.002，正相关）。此外，回归分析表明，（P = 0.005）传输效率和E（pd）总数之间（1）（t = 4.39，β = 0.45 +/- 0.10，r = 0.87）和波形E（pd）（1）的总持续时间（P = 0.05）（t = 4.00，β = 0.02 +/- 0.001，r = 0.94）。B成功接种TYLCV的最小韧皮部接触E（pd）（1）阈值期。烟粉虱对番茄植株的影响为1.8分钟。持续的病毒传播粉虱进行了讨论。
The feeding behavior of the whitefly Bemisia tabaci (Gennadius) was monitored using the electrical penetration graph (EPG) technique during the transmission process of tomato yellow leaf curl geminivirus (TYLCV). The behavior of individual viruliferous whiteflies was recorded on two-leaf stage tomato test plants (Lycopersicon esculentum Mill 'Riofuego'). A total of 213 whitefly individuals was recorded on single test plants during an inoculation access period that ranged from 3.5 to 14 h. Recordings were classified into 4 categories depending of the waveforms observed: group I, including only waveform C (pathway), was associated with a residual 2.4% TYLCV transmission efficiency; group II, showing stylet pathway and a single E(pd)(1) waveform, was associated with a 7.4% transmission efficiency; group III, showing stylet pathway and a single E(pd)(1) + E(pd)(2) waveform, achieved a 23.4% transmission efficiency; and finally group TV, showing pathway followed by several E(pd)(1) + E(pd)(2) waveforms, was associated with the highest transmission efficiency (37.5%). A total of 16 different behavioral variables was introduced into a stepwise-backward logistic regression model to determine the variables most related to TYLCV inoculation. Among them, the total duration of E(pd)(1) was the most significant variable associated with virus inoculation by B. tabaci (P = 0.002, positive relationship). In addition, the regression analysis indicated a strong positive relationship (P = 0.005) between transmission efficiency and total number of E (pd)(1) (t = 4.39, beta = 0.45 +/- 0.10, r = 0.87), and total duration of waveform E(pd)(1) (P = 0.05) (t = 4.00, beta = 0.02 +/- 0.001, r = 0.94). The minimum phloem contact E(pd)(1) threshold period observed for successful inoculation of TYLCV by B. tabaci on tomato plants was 1.8 min. Implications of these results for. persistent virus transmission by whiteflies are discussed.