FLUID REABSORPTION AND GLUCOSE CONSUMPTION IN EDEMATOUS RAT LUNGS
FLUID REABSORPTION AND GLUCOSE CONSUMPTION IN EDEMATOUS RAT LUNGS
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DOI：
10.1161/01.res.60.5.708
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发表时间：
1987-05-01
影响因子：
20.1
通讯作者：
HUKKANEN, J
中科院分区：
文献类型：
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作者：
EFFROS, RM;MASON, GR;HUKKANEN, J
溶质和水的摄取进行了研究，在离体灌流大鼠肺充满灌流液的空气空间。用伊文思蓝（T-1824）标记该溶液中的白蛋白，通过气道液中T-1824浓度增加6.5 ± 1.5来记录从气隙摄取液体。1.6%（n = 5，SEM），1小时和12.2 ±. 0.9%（n = 10）。可能导致肺泡液中液体损失的唯一可检测到的渗透力是气隙葡萄糖浓度的降低，其下降得更快（从150 mg/dl降至58.7 ± 0.001 mg/dl）。7.1 mg/dl，n = 10，2小时后）比血浆葡萄糖（从150 mg/dl至128.9 ±. 3.7 mg/dl）。添加5 ×10-5在1小时时，灌注液和空气空间溶液中的M特布他林几乎使液体重吸收加倍，普萘洛尔抑制了这种作用，并且似乎与葡萄糖消耗无关。暴露于特布他林2小时增加上皮细胞对3 H-甘露醇和22 Na+的通透性。这些观察结果表明，主动钠转运和上皮代谢或气道液中葡萄糖的转运可能各自在水肿液的重吸收中发挥作用。
Solute and water uptake were studied in isolated perfused rat lungs with airspaces filled with the perfusion fluid. The albumin in this solution was labelled with Evans blue (T-1824), and uptake of fluid from the airspaces was documented by an increase in T-1824 concentration in airway fluid of 6.5 .+-. 1.6% (n = 5, SEM) at 1 hour and 12.2 .+-. 0.9% (n = 10) at 2 hours. The only detectable osmotic force that could have contributed to a loss of fluid from the alveolar fluid was a decrease in airspace glucose concentrations, which fell much more rapidly (from 150 mg/dl to 58.7 .+-. 7.1 mg/dl, n = 10, after 2 hours) than plasma glucose (from 150 mg/dl to 128.9 .+-. 3.7 mg/dl). Addition of 5 .times. 10-5 M terbutaline to the perfusate and airspace solutions nearly doubled fluid reabsorption at 1 hour, an effect that was inhibited by propranolol and did not appear to be related to glucose consumption. Exposure to terbutaline for 2 hours increased epithelial permeability to 3H-mannitol and 22Na+. These observations suggest that active sodium transport and epithelial metabolism or transport of glucose in airway fluid may each play a role in the reabsorption of edema fluid.