Role of fungal antigens in eosinophilia-associated cellular responses in nasal
Role of fungal antigens in eosinophilia-associated cellular responses in nasal
复制标题
真菌抗原在鼻嗜酸性粒细胞增多相关细胞反应中的作用
DOI：
10.1111/j.1365-2222.2010.03584.x
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发表时间：
2011
影响因子：
6.1
通讯作者：
他
中科院分区：
文献类型：
--
作者：
Mitsuhiro Okano;Shin Kariya;他
Cite this as：M. Okano，T. Fujiwara，T. Haruna，S.卡里耶，S. Makihara，T. Higaki和K. Nishizaki，临床和实验过敏，2011（41）171- 178.综述背景真菌和/或金黄色葡萄球菌肠毒素（SE）可能参与慢性鼻窦炎伴鼻息肉（CRSwNP）病例中嗜酸性粒细胞炎症的发病机制。我们试图确定真菌抗原对鼻息肉中嗜酸性粒细胞相关细胞反应的影响。从13名CRSwNP患者中制备。DNPC与真菌提取物（曲霉属、链格孢属和念珠菌属）或SEB一起培养72小时，之后测量上清液中IL-5、IL-13和RANTES的水平。 还检查了对β-d-葡聚糖、甘露聚糖和甲壳素的反应。结果在200 μg/mL的曲霉菌作用下，分别有38.5%、69.2%和30.8%的DNPC产生IL-5、IL-13和RANTES。 在200 μg/mL链格孢菌作用下，分别有53.8%、53.8%和7.7%的DNPC产生IL-5、IL-13和RANTES。 200 μg/mL念珠菌作用于DNPCs，产生IL-5、IL-13和RANTES的细胞分别为53.8%、38.5%和15.4%。 所有DNPC响应于0.1 μg/mL SEB产生这些细胞因子。 SEB诱导的细胞因子水平显著高于真菌提取物。暴露于每种真菌提取物或SEB后细胞因子的产生与各种临床特征（包括鼻息肉嗜酸性粒细胞增多症和鼻窦炎的放射学严重程度）之间没有相关性。真菌致敏或支气管哮喘合并症均与真菌提取物诱导的DNPC细胞因子产生无关。β-d-葡聚糖，甘露聚糖和几丁质没有诱导显着的细胞因子productions.ConclusionsThese结果表明，虽然DNPC产生IL-5，IL-13和RANTES在响应真菌提取物，真菌抗原，包括主要的碳水化合物是不太能够诱导嗜酸性粒细胞增多相关的细胞反应，鼻息肉比SEB。
Cite this as: M. Okano, T. Fujiwara, T. Haruna, S. Kariya, S. Makihara, T. Higaki and K. Nishizaki, Clinical & Experimental Allergy, 2011 (41) 171–178.SummaryBackgroundFungi and/orStaphylococcus aureusenterotoxins (SEs) may participate in the pathogenesis of eosinophilic inflammation in cases of chronic rhinosinusitis with nasal polyps (CRSwNP).ObjectiveWe sought to determine the effects of fungal antigens on eosinophilia‐associated cellular responses in nasal polyps.MethodsDispersed nasal polyp cells (DNPCs) were prepared from 13 patients with CRSwNP. DNPCs were cultured with fungal extracts (Aspergillus, AlternariaandCandida) or SEB for 72 h, after which the levels of IL‐5, IL‐13 and RANTES were measured within the supernatant. Responses to β‐d‐glucan, mannan and chitin were also examined.Results38.5%, 69.2% and 30.8% of DNPCs produced IL‐5, IL‐13 and RANTES, respectively, in response to 200 μg/mL ofAspergillus. 53.8%, 53.8% and 7.7% of DNPCs produced IL‐5, IL‐13 and RANTES, respectively, in response to 200 μg/mL ofAlternaria. 53.8%, 38.5% and 15.4% of DNPCs produced IL‐5, IL‐13 and RANTES, respectively, in response to 200 μg/mL ofCandida. All DNPCs produced these cytokines in response to 0.1 μg/mL of SEB. SEB induced significantly greater cytokine levels than the fungal extracts. No correlation between cytokine production following exposure to each of the fungal extracts or SEB and various clinical features, including nasal polyp eosinophilia and radiological severity of sinusitis was observed. Neither sensitization to fungus nor comorbidity with bronchial asthma was correlated with the fungal extract‐induced cytokine production by DNPCs. β‐d‐glucan, mannan and chitin did not induce significant cytokine production.ConclusionsThese results suggest that, although DNPCs produce IL‐5, IL‐13 and RANTES in response to fungal extracts, fungal antigens including major carbohydrates are less capable of inducing eosinophilia‐associated cellular responses in nasal polyps than SEB.