Sulfated modification, characterization and antioxidant activities of polysaccharide from Cyclocarya paliurus
Sulfated modification, characterization and antioxidant activities of polysaccharide from Cyclocarya paliurus
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青钱柳多糖的硫酸化修饰、表征及抗氧化活性
DOI：
10.1016/j.foodhyd.2015.02.018
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发表时间：
2016-02-01
影响因子：
10.7
通讯作者：
Xie, Ming-Yong
中科院分区：
文献类型：
--
作者：
Xie, Jian-Hua;Wang, Zhi-Jun;Xie, Ming-Yong
本研究对青环己烷多糖进行化学改性，得到其硫酸化衍生物。采用化学方法、圆二色光谱法、高效阴离子交换色谱法、高效凝胶渗透色谱法、傅立叶变换红外光谱法、羟基自由基法、DPPH自由基法、超氧化物自由基法和β -胡萝卜烯油酸法，对这些硫酸酸化多糖衍生物的理化性质和抗氧化性能进行了比较研究。结果表明，这些改性是成功的，磺化后多糖的理化性质和抗氧化性能都发生了变化。硫酸化多糖中，取代度（DS）最低、分子量（Mw）和蛋白质含量最高的S-CP1-8在羟基、超氧化物、DPPH自由基和b-胡萝卜烯油酸试验中表现出优异的抗氧化活性。综上所述，绿环子硫酸酸化多糖的体外抗氧化活性可能与DS、Mw、硫酸盐含量、蛋白质和醛酸含量的综合作用有关。(C) 2015 Elsevier Ltd.版权所有。
In this study, polysaccharide extracted from Cyclocarya paliurus was chemically modified to obtain its sulfated derivatives. The physicochemical characterizations and antioxidant properties of these sulfated polysaccharide derivatives were comparatively investigated by chemical methods, circular dichroism spectra, high-performance anion exchange chromatography, high-performance gel permeation chromatography, fourier transform infrared spectrometry, and hydroxyl radical assay, DPPH radical assay, superoxide radical assay and beta-caroteneelinoleic acid assay, respectively. Results showed that these modifications were successful, and the physicochemical and antioxidant properties of polysaccharides were changed after sulfation. Among these sulfated polysaccharides, the S-CP1-8 with relatively the lowest degree of substitution (DS), molecular weight (Mw) and the highest protein content exhibited excellent antioxidant activities in hydroxyl, superoxide, DPPH radical assay and b-caroteneelinoleic acid assay. These results indicated that the in vitro antioxidant activities of the sulfated polysaccharides from Cyclocarya paliuru may be related to combined effects of DS, Mw, sulfate content, protein and uronic acid content. (C) 2015 Elsevier Ltd. All rights reserved.