GASTROINTESTINAL COLONIZATION AND SYSTEMIC DISSEMINATION BY CANDIDA-ALBICANS AND CANDIDA-TROPICALIS IN INTACT AND IMMUNOCOMPROMISED MICE
GASTROINTESTINAL COLONIZATION AND SYSTEMIC DISSEMINATION BY CANDIDA-ALBICANS AND CANDIDA-TROPICALIS IN INTACT AND IMMUNOCOMPROMISED MICE
复制标题
DOI：
10.1128/iai.60.11.4907-4914.1992
复制
发表时间：
1992-11-01
影响因子：
3.1
通讯作者：
MATHIEU, LG
中科院分区：
文献类型：
--
作者：
DEREPENTIGNY, L;PHANEUF, M;MATHIEU, LG
比较了白色念珠菌和热带念珠菌在正常小鼠和免疫功能受损小鼠的胃肠道定植和全身传播。用急性白血病患者血液中分离的2株白色念珠菌和2株热带念珠菌1.0 × 10(7) CFU，接种5日龄CFW小鼠，同时接种白色念珠菌4918及其耐蓝蛋白突变体4918-10。白色念珠菌和热带念珠菌在接种后30分钟内扩散到肺、肝脏和肾脏，在随后的10天内，两种物种的器官CFU具有可比性。胚分生孢子与小肠绒毛表面的紧密结合导致微绒毛的溶解，然后逐渐侵入绒毛。绒毛内的囊胚分生孢子周围有明显的间隙区。接种后31天内，白色念珠菌血分离株和菌株4918、4918-10在小肠和大肠的持续定植量相似，但始终超过热带念珠菌。在白色念珠菌定植的小鼠中，接种后第30和33天使用醋酸可的松和环磷酰胺免疫抑制可使胃CFU增加40- 370倍，使肠道CFU增加30- 80倍。相比之下，在免疫抑制之前，热带梭菌的持续定植是无法检测到的，只有在治疗后才变得明显。白色念珠菌比热带念珠菌播散更频繁，器官CFU也更高。尽管如此，感染热带梭菌的小鼠中有20%死亡，而感染白色梭菌血液分离株的小鼠中只有4%死亡。间接免疫荧光显示念珠菌菌丝仅在免疫抑制、持续定植的小鼠胃粘膜和粘膜下层中穿透生长。综上所述，这些数据表明，热带梭菌似乎比白色梭菌更具毒性，导致免疫功能受损小鼠胃肠道定植、全身传播和死亡的因素可能并不相同。
Gastrointestinal colonization and systemic dissemination by Candida albicans and Candida tropicalis were compared in intact and immunocompromised mice. Five-day-old CFW mice were inoculated by the oral-intragastric route with 1.0 X 10(7) CFU of two C albicans and two C tropicalis strains isolated from the blood of patients with acute leukemia and with C albicans 4918 and its cerulenin-resistant mutant 4918-10. C albicans and C tropicalis spread to the lungs, liver, and kidneys within 30 min postinoculation, and organ CFU of the two species were comparable over the following 10 days. Close association of blastoconidia with the villous surface of the small intestine resulted in lysis of microvilli and then progressive invasion of villi. Blastoconidia within villi were surrounded by a conspicuous zone of clearing. Persistent colonization of the small and large intestines by C. albicans blood isolates and strains 4918 and 4918-10 was similar for 31 days after inoculation, but consistently exceeded that of C. tropicalis. In mice colonized with C. albicans, immunosuppression with cortisone acetate and cyclophosphamide on days 30 and 33 after inoculation increased stomach CFU 40- to 370-fold and intestinal CFU 30- to 80-fold. In contrast, persistent colonization by C. tropicalis was undetectable before immunosuppression and only became apparent after treatment. C. albicans disseminated more frequently and with higher organ CFU than C. tropicalis. Despite this fact, 20% of mice infected with C. tropicalis died, compared with 4% infected with C. albicans blood isolates. Indirect immunofluorescence revealed penetrative growth by Candida hyphae exclusively in the mucosa and submucosa of the stomach from immunosuppressed, persistently colonized mice. Taken together, the data indicate that C. tropicalis appears to be more virulent than C. albicans and that factors responsible for gastrointestinal colonization, systemic dissemination, and mortality in immunocompromised mice may not be identical.