Accelerated bone repair with the use of a synthetic BMP-2-derived peptide and bone-marrow stromal cells
Accelerated bone repair with the use of a synthetic BMP-2-derived peptide and bone-marrow stromal cells
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DOI：
10.1002/jbm.a.30208
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Tanihara, M
中科院分区：
文献类型：
--
作者：
Saito, A;Suzuki, Y;Tanihara, M
一种新的合成肽对应BMP-2残基73-92，可以诱导骨形成，并可以与载体形成缀合物以定位其作用，此前已有报道。合成的肽与bmp -2特异性受体结合，可提高小鼠多能间充质细胞系C3H10T1/2的碱性磷酸酶活性和骨钙素mRNA水平。当73-92肽与共价交联海藻酸盐凝胶结合并植入大鼠小腿肌肉时，也能诱导异位骨形成。本研究报道73-92肽偶联藻酸盐凝胶颗粒显著促进大鼠胫骨骨缺损的修复，而肽偶联藻酸盐凝胶海绵效果较差。当73-92肽结合海藻酸盐凝胶颗粒与同系大鼠骨髓基质细胞共植入时，骨再生速度进一步加快，密度更高。因此，73-92肽能诱导成骨前体细胞向成骨细胞分化，并能激活成骨细胞，促进骨缺损的修复。(C) 2004 Wiley期刊有限公司
A novel synthetic peptide corresponding to BMP-2 residues 73-92 that can induce bone formation and can form a conjugate with a carrier to localize its effect has been reported previously. The synthetic peptide was bound to a BMP-2-specific receptor, and it elevated both the alkaline phosphatase activity and the osteocalcin mRNA in the murine multipotent mesenchymal cell line, C3H10T1/2. The 73-92 peptide also induced ectopic bone formation when conjugated to a covalently crosslinked alginate gel and implanted into a rat's calf muscle. Here, it is reported that the 73-92 peptide-conjugated alginate gel particles significantly promoted the repair of rat tibial bone defects, whereas the alginate gel sponge that the peptide was conjugated with was less effective. Further acceleration and denser bone regeneration was achieved when the 73-92 peptide-conjugated alginate gel particles were coimplanted with syngeneic rat bone-marrow stromal cells. Therefore, the 73-92 peptide can induce differentiation of osteoblast precursor cells into osteoblasts, and can activate osteoblasts to promote the repair of bone defects. (C) 2004 Wiley Periodicals, Inc.