Lysozyme gene expression by hemocytes of Pacific white shrimp, Litopenaeus vannamei, after injection with Vibrio
Lysozyme gene expression by hemocytes of Pacific white shrimp, Litopenaeus vannamei, after injection with Vibrio
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DOI：
10.1016/j.fsi.2006.06.004
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发表时间：
2007-04-01
影响因子：
4.7
通讯作者：
Burnett, Karen G.
中科院分区：
文献类型：
--
作者：
Burge, Erin J.;Madigan, Daniel J.;Burnett, Karen G.
本研究旨在量化凡纳滨对虾（Litopenaeus vannamei Boone）组织中溶菌酶（一种由虾血细胞产生的重要抗菌蛋白）的基因表达，以应对病原体的挑战。我们用实时PCR方法定量了溶菌酶转录物，并使用这些数据和血细胞总数来推断免疫反应期间血细胞运输的模式。通过原位杂交检测mRNA在循环血细胞和高血细胞浓度组织中的转录表达。用鲍曼弧菌攻毒后48 h，监测5个组织和循环血细胞中溶菌酶基因的表达。结果表明溶菌酶在循环中的大部分血细胞和周围组织中表达。注射canipbelllii后4小时，循环血细胞和外周组织的转录信号明显降低。在相同的早期阶段，溶菌酶信号在注射部位的肌肉中显著增加，并在整个时间过程中保持高水平，这表明在免疫反应过程中，血细胞在早期被募集到注射部位。4小时后，循环血细胞和组织中的溶菌酶信号增加，除注射部位的肌肉外，所有组织均恢复到控制水平。(c) 2006 Elsevier Ltd.版权所有。
The purpose of this study was to quantify the gene expression of lysozyme, an important antibacterial protein produced by shrimp hemocytes, within tissues of Litopenaeus vannamei Boone in response to a pathogen challenge. We quantified lysozyme transcripts with a real-time PCR method and used these data, along with total hemocyte counts, to infer patterns of hemocyte trafficking during the immune response. Transcript expression was detected by in situ hybridization of mRNA in circulating hemocytes, and within tissues with high hemocyte concentrations. Lysozyme gene expression was monitored in 5 tissues and in circulating hemocytes for 48 h following challenge with the shrimp pathogen Vibrio canipbellii Baumann. The results suggest that lysozyme is expressed in most if not all hemocytes in circulation and in peripheral tissues. Injection with V. canipbellii produced a significant decrease in transcriptional signal in circulating hemocytes and peripheral tissues 4 h after injection. Over the same early time period lysozyme signal increased significantly in the muscle at the site of injection and remained high for the duration of the time-course, suggesting that hemocytes are recruited to the site of injection early during the course of the immune response. After 4 h, lysozyme signal increased in circulating hemocytes and tissues, with a return to control levels noted for all tissues except the muscle at the site of injection. (c) 2006 Elsevier Ltd. All rights reserved.