IDENTIFICATION OF A T-CELL EPITOPE ADJACENT TO NEUTRALIZATION ANTIGENIC SITE-1 OF POLIOVIRUS TYPE-1
IDENTIFICATION OF A T-CELL EPITOPE ADJACENT TO NEUTRALIZATION ANTIGENIC SITE-1 OF POLIOVIRUS TYPE-1
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DOI：
10.1128/jvi.65.2.711-718.1991
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发表时间：
1991-02-01
影响因子：
5.4
通讯作者：
VANDERWERE, S
中科院分区：
文献类型：
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作者：
LECLERC, C;DERIAUD, E;VANDERWERE, S
通过使用灭活的纯化病毒或衣壳蛋白 VP1 合成肽，分析了不同品系小鼠中对脊髓灰质炎病毒的增殖性 T 细胞反应。 用灭活的 1 型脊髓灰质炎病毒 (PV1) 免疫小鼠后，在用纯化病毒进行体外刺激后，其淋巴结 CD4+ T 细胞出现特异性增殖反应。 在用 PV1、PV2 或 PV3 免疫的小鼠中，在用异源病毒进行体外刺激后，观察到 T 细胞反应的强烈交叉反应性。 通过使用各种策略，在衣壳多肽 VP1 的氨基酸 103 至 115 区域中鉴定出一个显性 T 细胞表位，靠近 C3 中和表位。 T 细胞对 VP1 氨基酸 103 至 115 的反应受 H-2 限制：H-2d 小鼠是反应者，而 H-2k 和 H-2b 小鼠对此 T 细胞表位不反应。 用未偶联的 p86-115 肽（代表 VP1 氨基酸 86 至 115，包含 T 细胞表位和 C3 中和表位）对 BALB/c (H-2d) 小鼠进行免疫，诱导脊髓灰质炎病毒特异性 B 和 T 细胞反应。 此外，这些小鼠产生了脊髓灰质炎病毒中和抗体。
Proliferative T-cell responses to poliovirus in various strains of mice have been analyzed by using either killed purified virus or capsid protein VP1 synthetic peptides. Following immunization of mice with inactivated poliovirus type 1 (PV1), a specific proliferative response of their lymph node CD4+ T cells was obtained after in vitro stimulation with purified virus. In mice immunized with PV1, PV2, or PV3, a strong cross-reactivity of the T-cell responses was observed after in vitro stimulation with heterologous viruses. By using various strategies, a dominant T-cell epitope was identified in the amino acid 103 to 115 region of capsid polypeptide VP1, close by the C3 neutralization epitope. The T-cell response to VP1 amino acids 103 to 115 is H-2 restricted: H-2d mice are responders, whereas H-2k and H-2b mice do not respond to this T-cell epitope. Immunization of BALB/c (H-2d) mice with the uncoupled p86-115 peptide, which represents VP1 amino acids 86 to 115 and contains both the T-cell epitope and the C3 neutralization epitope, induced poliovirus-specific B- and T-cell responses. Moreover, these mice developed poliovirus neutralizing antibodies.