Effects of hydrogen sulfide on hypoxic pulmonary vascular structural remodeling
Effects of hydrogen sulfide on hypoxic pulmonary vascular structural remodeling
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硫化氢对缺氧肺血管结构重塑的影响。
DOI：
10.1016/j.lfs.2005.07.009
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发表时间：
2006-02-16
期刊：
影响因子：
6.1
通讯作者：
Du, JB
中科院分区：
文献类型：
--
作者：
Jin, HF;Cong, BL;Du, JB
为探讨硫化氢（H2S）在缺氧性肺血管结构重建（HPVSR）中的作用，将24只Wistar大鼠随机分为3组：对照组（n=8）、缺氧组（n=8）和缺氧加硫氢化钠（hy+NaHS）组（n=8）。测定平均肺动脉压（mPAP）、血浆硫化氢（H2S）及肺小血管中肌化动脉（MA）、部分肌化动脉（PMA）和非肌化动脉（NMA）的百分比。免疫组化法检测I型胶原、111型胶原、弹性蛋白、转化生长因子β 3（TGF-β 3）、增殖细胞核抗原（PCNA）和人尾加压素II（U-II）的表达。采用原位杂交法检测Ⅰ、Ⅲ型前胶原、基质金属蛋白酶-1（MMP-1）和金属蛋白酶组织抑制剂-1（TIMP-1）mRNA的表达。结果表明，NaHS可显著增加低氧大鼠血浆H_2S含量，降低mPAP，降低肺小血管MA和PMA的百分比。NaHS可抑制肺动脉平滑肌细胞（PASMCs）的增殖，表现为肺动脉壁PCNA和人U-Ⅱ的表达减少。NaHS可降低缺氧大鼠肺动脉I、III型胶原、弹性蛋白和TGF-β 3蛋白的表达，降低I、III型前胶原mRNA的表达，但对TIMP-1/MMP-1 mRNA的比值无影响。提示H2S在HPVSR的发生发展中起重要作用。(c)2005年爱思唯尔公司All rights reserved.
study the role of hydrogen sulfide (H2S) in hypoxic pulmonary vascular structural remodeling (HPVSR), a total of 24 Wistar rats were randomly divided into three groups: control group (n=8), hypoxia group (n=8) and hypoxia with sodium hydrosulfide (hy+NaHS) group (n=8). The mean pulmonary artery pressure (mPAP), plasma H2S and the percentage of muscularized arteries (MA), partially muscularized arteries (PMA) and nonmuscularized arteries (NMA) in small pulmonary vessels were measured. Collagen I and 111, elastin, transforming growth factor-beta(3) (TGF-beta(3)), proliferative cell nuclear antigen (PCNA) and human urotensin II(U-II) expressions were detected by immunohistochemical assay. The mRNA expressions of procollagen I and III, matrix metalloproteinase-1 (MMP-1) and tissue inhibitor of metalloproteinease-1 (TIMP-1) were detected by in situ hybridization. The results showed that NaHS significantly increased plasma H2S, decreased mPAP and the percentage of MA and PMA of small pulmonary vessels in rats under hypoxia. Meanwhile, NaHS inhibited the proliferation of pulmonary artery smooth muscle cells (PASMCs) represented by a decrease in the expressions of PCNA and human U-II in pulmonary artery wall. NaHS reduced the expression of collagen I and III, elastin and TGF-beta(3) protein and decreased the expressions of procollagen I and III mRNA in pulmonary arteries of rats under hypoxia, but it did not impact the ratio of TIMP-1 mRNA to MMP-1 mRNA in pulmonary arteries of rats under hypoxia. These data suggested that H2S played an important role in the development of HPVSR. (c) 2005 Elsevier Inc. All rights reserved.