Immunodetection of human T-cell lymphotropic virus type I core protein in biological samples by using a monoclonal antibody immunoassay.
Immunodetection of human T-cell lymphotropic virus type I core protein in biological samples by using a monoclonal antibody immunoassay.
复制标题
使用单克隆抗体免疫分析法对生物样品中的人 T 细胞嗜淋巴细胞病毒 I 型核心蛋白进行免疫检测。
DOI：
10.1128/jcm.28.5.949-955.1990
复制
发表时间：
1990
影响因子：
9.4
通讯作者：
Poiesz,B
中科院分区：
文献类型：
--
作者：
Papsidero,L;Swartzwelder,F;Sheu,M;Montagna,R;Ehrlich,G;Bhagavati,S;Dosik,H;Sninsky,J;Poiesz,B
建立了一种检测人嗜T淋巴细胞病毒I型（HTLV-I）核心蛋白的单克隆抗体酶免疫分析法（EIA）。单克隆抗体（克隆6.11）特异性识别病毒的p19 gag基因编码蛋白。EIA比逆转录酶测量灵敏100倍以上，并且能够响应小于500 pg的全病毒裂解物。该测定法表现出类型特异性，因为HTLV-II抗原不能产生阳性信号。此外，一组其他病毒显示无抗原交叉反应性。这些病毒包括疱疹病毒、麻疹病毒、人类免疫缺陷病毒等。在去污剂存在下，在密度梯度超离心过程中跟踪病毒p19，发现其与病毒膜蛋白相关。相比之下，逆转录酶活性定位在含有无核芯的较高密度的组分中。临床上，EIA用于检测HTLV-I相关脊髓病患者和前病毒整合的无病毒感染个体的病毒培养物中的HTLV-I抗原。
A monoclonal antibody-based enzyme immunoassay (EIA) has been developed for detection of human T-cell lymphotropic virus type I (HTLV-I) core protein. The monoclonal antibody (clone 6.11) specifically recognizes the p19 gag gene-encoded protein of the virus. The EIA was over 100 times more sensitive than reverse transcriptase measurement and was capable of responding to less than 500 pg of whole-virus lysate. The assay exhibited type specificity in that HTLV-II antigens failed to produce a positive signal. In addition, a panel of other viruses demonstrated no antigenic cross-reactivity. These included herpesviruses, measles virus, human immunodeficiency viruses, and others. Viral p19 was followed during the course of density gradient ultracentrifugation in the presence of detergent, where it was noted to associate with viral membrane proteins. In comparison, reverse transcriptase activity localized in fractions of higher density containing envelope-free cores. Of clinical interest, the EIA was used to detect HTLV-I antigen in the viral cultures of patients with HTLV-I-associated myelopathies and from symptom-free individuals with proviral integration.