The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
The Cutting and Floating Method for Paraffin-embedded Tissue for Sectioning
复制标题
石蜡包埋组织切片的切割和漂浮方法
DOI：
10.3791/58288
复制
发表时间：
2018-09-01
影响因子：
1.2
通讯作者：
Zou, Suqi
中科院分区：
文献类型：
--
作者：
Qin, Cheng;Bai, Yijiang;Zou, Suqi
石蜡包埋组织的切片广泛用于组织学和病理学。然而，它是乏味的。为了改进这种方法，几家商业公司已经设计出使用流体水的复杂的切片转移系统。为了简化这项技术，我们创造了一种使用自制设备的简单方法，该设备将切割和漂浮结合在一个简单的水槽中;因此，切片自动进入水面上的水浴。采用常规石蜡切片法和本方法对成年小鼠脑、成年小鼠肾、胚胎小鼠脑和成年斑马鱼眼的海马进行了切片比较。统计分析表明，我们的改进方法节省了时间，并产生了更高质量的切片。此外，对于初级操作员来说，在短时间内对整个标本进行石蜡切片是很容易的。
Sectioning of the paraffin-embedded tissue is widely used in histology and pathology. However, it is tedious. To improve this method, several commercial companies have devised complex section transfer systems using fluid water. To simplify this technology, we created a simple method using homemade equipment that combines cutting and floating within a simple thermostatic chamber; therefore, the sections automatically enter the water bath on the water surface. The hippocampus from adult mouse brains, adult mouse kidneys, embryonic mouse brains, and adult zebrafish eyes were cut using both conventional paraffin sectioning and the presented method for comparison. Statistical analysis shows that our improved method saved time and produced higher quality sections. In addition, paraffin sectioning of a whole specimen in a short time is easy for junior operators.