Anti-diabetic activities of agaropectin-derived oligosaccharides from Gloiopeltis furcata via regulation of mitochondrial function
Anti-diabetic activities of agaropectin-derived oligosaccharides from Gloiopeltis furcata via regulation of mitochondrial function
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来自分叉柳树的琼脂胶衍生寡糖通过调节线粒体功能的抗糖尿病活性
DOI：
10.1016/j.carbpol.2019.115482
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发表时间：
2020
影响因子：
11.2
通讯作者：
Yu Guangli
中科院分区：
文献类型：
--
作者：
Wang Xueliang;Jiang Hao;Zhang Ning;Cai Chao;Li Guoyun;Hao Jiejie;Yu Guangli
本研究旨在探讨海萝寡糖（SAOs）对棕榈酸钠（PA）诱导的胰岛素抵抗HepG 2细胞是否具有抗糖尿病作用。我们发现SAO与线粒体共定位并调节线粒体功能。SAO降低呼吸链活性，导致呼吸耗氧量降低，ADP/ATP比值升高，呈一定的剂量依赖性。因此，SAO减轻PA处理的细胞中的氧化应激状态，此外，同时调节ROS-JNK-IRS-1通路。因此，SAO通过激活IRS-1-AKT-GSK-3β-GS通路增强胰岛素敏感性和葡萄糖代谢。此外，SAO通过PKA-LKB 1和细胞调节的能量代谢途径激活AMPK。因此，SAO通过调节HepG 2细胞中HMGCR、ACC和SREBP-1蛋白的表达，减少脂质的积累，改善脂质代谢。综上所述，我们得出结论，SAOs可以通过调节线粒体及其下游信号通路来显著改善体外糖尿病状态。
The aim of the present study was to investigate whether agaropectin-derived oligosaccharides fromGloiopeltis furcata(SAOs) exert an anti-diabetic effect in sodium palmitate (PA)-induced insulin resistant HepG2 cells. We found that SAOs were co-localized with mitochondria and regulated mitochondrial function. SAOs reduced respiratory chain activities, which led to reduced respiratory oxygen consumption and increased the cellular ADP/ATP ratio in a certain degree of dose-dependent manner. Thus, SAOs alleviated the oxidative stress state in PA-treated cells and, moreover, concurrently regulated the ROS-JNK-IRS-1 pathway. As a result, SAOs enhanced insulin sensitivity and glucose metabolism by activating the IRS-1-AKT-GSK-3β-GS pathway. Additionally, SAOs activated AMPK through both PKA-LKB1 and mitochondrial-regulated energy metabolism pathways. Therefore, SAOs decreased accumulation of lipids and improved lipid metabolism via regulating HMGCR, ACC and SREBP-1 proteins in HepG2 cells. Taken together, we conclude that SAOs could significantly ameliorate diabetic statesin vitrovia regulating mitochondria and their downstream signaling pathways.