RNAi-Based Transcriptome Suggests Candidate Genes Regulated by ZxNHX1 to Affect The Salt Tolerance of Zygophyllum xanthoxylum
RNAi-Based Transcriptome Suggests Candidate Genes Regulated by ZxNHX1 to Affect The Salt Tolerance of Zygophyllum xanthoxylum
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基于RNAi的转录组表明ZxNHX1调控的候选基因影响霸王的耐盐性
DOI：
10.1007/s00344-021-10460-w
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发表时间：
2021-08-02
影响因子：
4.8
通讯作者：
Wang，Suo-Min
中科院分区：
文献类型：
--
作者：
Liu，Hai-Shuang;Guo，Xiao-Nong;Wang，Suo-Min
霸王（Zygophyllum xanthoxylum）是一种多汁旱生植物，具有优异的耐盐性，其耐盐性与ZxNHX 1介导的液泡Na +区室化密切相关。RNA干扰（RNAi）介导的ZxNHX 1基因沉默可使ZxNHX 1基因的Na +积累特性受损，从而抑制ZxNHX 1基因的正常生长。花椒为了探讨盐胁迫下ZxNHX 1-RNAi转基因植株表型改变的分子机制，我们在转录组水平上比较了ZxNHX 1-RNAi转基因植株与野生型转基因植株中盐胁迫相关基因的表达差异。结果表明，在对照和盐处理条件下，ZxNHX 1-RNAi株系与野生型之间有大量基因在叶片和根中表达差异。其中142个单基因在50 mM NaCl处理6 h和24 h后，ZxNHX 1-RNAi株系和野生型之间在根和叶中均有不同程度的表达。这些差异表达的基因（DEG）与跨膜转运的基础上基因本体论（GO）注释。在离子转运方面，共鉴定出49个DEG，其中一些与Na +和K +稳态相关的基因被下调;同时，ZxNHX 1的沉默触发了参与重要营养元素（包括N，P，Ca和Mg）转运的几个基因的差异表达。此外，ZxNHX 1基因的沉默分别影响了18个和26个与光合作用和活性氧清除相关的基因的表达水平。本研究为ZxNHX 1在维持盐积累特性和调节盐胁迫下的耐盐性方面发挥主导作用提供了有力的证据。花椒
Zygophyllum xanthoxylum , a succulent xerophyte, possesses excellent salt tolerance which is closely associated with vacuolar Na + compartmentation via ZxNHX1. RNA interference (RNAi)-mediated ZxNHX1 silencing impaired the characteristics of Na + accumulation and thus inhibited normal growth of Z. xanthoxylum . To explore the molecular mechanisms underlying the changed phenotype, here we compared the different expression of salt-responsive genes between ZxNHX1 -RNAi line and wild type at transcriptome level. The result showed that vast genes were differently expressed in leaves or roots between ZxNHX1 -RNAi line and wild type under control condition or salt treatment. Among them, 142 unigenes were differently expressed in both roots and leaves under 50 mM NaCl for 6 h and 24 h between ZxNHX1 -RNAi line and WT. These differentially expressed genes (DEGs) were related to transmembrane transportation based on Gene Ontology (GO) annotations. In terms of ion transport, 49 DEGs were identified, in which some genes associated with Na + and K + homeostasis were down-regulated; meanwhile, silencing of ZxNHX1 triggered the differential expression of several genes involved in the transport of important nutrient elements including N, P, Ca, and Mg. Besides, silencing of ZxNHX1 affected the expression level of 18 and 26 genes related to photosynthesis and ROS scavenging, respectively. This study provided strong evidences to indicate the dominant role of ZxNHX1 in maintaining the characteristics of salt accumulation and regulating the salt tolerance of Z. xanthoxylum .