SEPARATION OF HUMAN BREAST-CANCER CELLS FROM BLOOD BY DIFFERENTIAL DIELECTRIC AFFINITY
SEPARATION OF HUMAN BREAST-CANCER CELLS FROM BLOOD BY DIFFERENTIAL DIELECTRIC AFFINITY
复制标题
DOI：
10.1073/pnas.92.3.860
复制
发表时间：
1995-01-31
影响因子：
11.1
通讯作者：
GASCOYNE, PRC
中科院分区：
文献类型：
--
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
电旋转测量表明，转移性人乳腺癌细胞系MDA231的介电性质与红细胞和T淋巴细胞的介电性质有显著不同。利用这些介电差异，通过适当地平衡作用于包含微电极阵列的介电亲和柱内细胞的流体动力和电泳力，将癌细胞与正常血细胞分开。分析了在这样的柱中成功分离颗粒的操作标准，我们的发现表明，介电亲和技术可能被证明在广泛的细胞分离和表征应用中是有用的。
Electrorotation measurements were used to demonstrate that the dielectric properties of the metastatic human breast cancer cell line MDA231 were significantly different from those of erythrocytes and T lymphocytes. These dielectric differences were exploited to separate the cancer cells from normal blood cells by appropriately balancing the hydrodynamic and dielectrophoretic forces acting on the cells within a dielectric affinity column containing a microelectrode array. The operational criteria for successful particle separation in such a column are analyzed and our findings indicate that the dielectric affinity technique may prove useful in a wide variety of cell separation and characterization applications.