Protective Effects of Akebia Saponin D Against Rotenone-Induced Hepatic Mitochondria Dysfunction
Protective Effects of Akebia Saponin D Against Rotenone-Induced Hepatic Mitochondria Dysfunction
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DOI：
10.1254/jphs.14135fp
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发表时间：
2014-11-01
影响因子：
3.5
通讯作者：
Liu, Li-hong
中科院分区：
文献类型：
--
作者：
Gong, Li-li;Wang, Zi-hui;Liu, Li-hong
木通皂苷D（Akebia saponin D，ASD）是从川断属植物川断（Dipsacus asper Wall）根茎中提取的一种具有生物活性的三萜皂苷。先前的研究已经发现ASD在小鼠模型中具有保肝作用。本研究旨在探讨ASD对BRL细胞和离体大鼠肝线粒体保护作用的分子机制。我们研究了ASD对鱼藤酮诱导的BRL细胞毒性的影响。结果表明，ASD抑制活性氧化物的积累，ATP缺乏和线粒体膜电位耗散;改善线粒体呼吸功能障碍，并以浓度依赖性方式提高复合物I的活性，表明ASD可能改善线粒体功能。ASD抑制鱼藤酮诱导的BRL细胞凋亡，并增加Bcl-2/Bax比值。这些结果表明，ASD可能通过线粒体对鱼藤酮诱导的毒性发挥肝保护作用。本研究支持了我们前期的研究，即ASD在体内具有肝保护作用，值得进一步研究。
Akebia saponin D (ASD) is a typical bioactive triterpenoid saponin obtained from the rhizome of Dipsacus asper Wall. Previous studies have found that ASD has a hepatoprotective effect in a mouse model. The purpose of this paper was to explore the molecular mechanism of the hepatoprotective effects of ASD on BRL cells and isolated rat liver mitochondria. We investigated the effects of ASD on rotenone-induced toxicity in BRL cells. The results showed that ASD inhibited the accumulation of reactive oxidant species, ATP deficiency, and mitochondrial membrane potential dissipation; ameliorates mitochondrial respiratory dysfunction, and improved the activity of complex I in a concentration-dependent manner, indicating that ASD likely improved mitochondrial function. ASD suppressed rotenone-induced BRL cell apoptosis and increased Bcl-2/Bax ratio. These results suggest that ASD may exert hepatoprotective effects against rotenone-induced toxicity through mitochondria. This study supports our previous research that ASD possesses hepatoprotective activity in vivo and it is worthy of further study.