The ultrastructural organization of the patch matrix compartments in the human striatum.
The ultrastructural organization of the patch matrix compartments in the human striatum.
复制标题
人类纹状体中斑块基质隔室的超微结构组织。
DOI：
10.1002/cne.10351
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发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Knickman,JoyK
中科院分区：
文献类型：
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作者：
Roberts,RosalindaC;Knickman,JoyK
哺乳动物纹状体是一种异质结构，其特征在于纹状体和基质。在包括人类在内的多种哺乳动物物种中，纹状体的突触组织已经被描述过，然而，纹状体组织中潜在的超微结构差异还没有得到很好的研究。从马里兰州脑收集中心获得纹状体组织样品（n = 7）（平均年龄，37.7 ± 9.4岁;平均PMI，5.3 ± 1.4小时）。制备组织用于钙结合蛋白免疫细胞化学以鉴定纹状体（斑块）和纹状体外基质（基质）隔室，随后制备用于电子显微镜检查。突触密度测定，使用体视学方法，所有的突触组合和突触的各种子集，如不对称，对称，轴棘，轴树突，穿孔的补丁和基质的尾状核（CP，CM）和壳核（PP，PM）。ANOVA显示以下类型突触的组间（CP、CM、PP、PM）差异显著（P< 0.05）：总组合、不对称、轴棘和不对称轴棘。这四种类型中的每一种在CP与PP中的密度显著增加，而基质（CM与PM）在这些或其他突触中的密度没有显着差异。在尾状核（CP与CM），研究的突触类型的突触密度没有显着变化之间的补丁和矩阵。在壳核，总突触，对称树突和穿孔的矩阵（PM）有较高的突触密度比补丁（PP）。这些数据表明，补丁和基质室是异质性的超微结构水平，赋予另一个层次的复杂性纹状体的事实，应该考虑到在电子显微镜水平研究疾病的大脑区域。J. Comp.神经元452：128-138，2002.© 2002 Wiley利斯公司
The mammalian striatum is a heterogeneous structure characterized by striosomes and matrix. The synaptic organization of the striatum has been described previously in various mammalian species including human; however, potential ultrastructural differences in striosomal organization have not been well studied. Samples (n = 7) of striatal tissue were obtained from the Maryland Brain Collection (mean age, 37.7 ± 9.4 years; and mean PMI, 5.3 ± 1.4 hours). Tissue was prepared for calbindin immunocytochemistry to identify striosomal (patch) and extrastriosomal matrix (matrix) compartments and subsequently prepared for electron microscopy. Synaptic density was determined, using stereologic methods, for all synapses combined and for various subsets of synapses such as asymmetric, symmetric, axospinous, axodendritic, and perforated in the patch and matrix of the caudate (CP, CM) and putamen (PP, PM). An ANOVA revealed significant between‐group (CP, CM, PP, PM) differences (P< 0.05) for the following types of synapses: total combined, asymmetric, axospinous, and asymmetric axospinous. Each of these four types was significantly increased in density in the CP vs the PP, whereas the matrix (CM vs PM) showed no significant differences in density in these or other synapses. In the caudate (CP vs CM), the synaptic density of the types of synapses studied did not vary significantly between the patch and the matrix. In the putamen, the matrix (PM) had higher synaptic densities than that of the patches (PP) for total synapses, symmetric dendritic, and perforated. These data show that the patch and matrix compartments are heterogeneous at the ultrastructural level, imparting another level of complexity to the striatum—a fact that should be taken into consideration when studying diseases of this brain region at the electron microscopic level. J. Comp. Neurol. 452:128–138, 2002. © 2002 Wiley‐Liss, Inc.