Chronic social stress in pigs impairs intestinal barrier and nutrient transporter function, and alters neuro-immune mediator and receptor expression.
Chronic social stress in pigs impairs intestinal barrier and nutrient transporter function, and alters neuro-immune mediator and receptor expression.
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DOI：
10.1371/journal.pone.0171617
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Moeser AJ
中科院分区：
文献类型：
--
作者：
Li Y;Song Z;Kerr KA;Moeser AJ
心理社会应激是人类和动物胃肠道(GI)病理生理学和疾病易感性的主要驱动因素。关于应激引起的胃肠道疾病的易感性机制仍然知之甚少。本研究探讨了7d慢性混合/拥挤应激对猪肠道屏障和营养物质转运功能、促肾上腺皮质激素释放因子(CRF)信号转导和免疫反应的影响。这项研究的结果表明，CS导致回肠和结肠屏障功能的显着损害，表现为回肠跨上皮电阻(TER)降低，回肠和结肠FD4流量增加(0.8倍)和0.7倍(0.7倍)。回肠钠葡萄糖连接转运体1(SGLT-1)功能，以葡萄糖诱导的短路电流(ISC)变化衡量，在CS猪中降低(52%)，与体重增加和饲料效率降低相关。虽然在CS猪中观察到SGLT-1功能的降低，但在CS猪中SGLT-1的mRNA表达和绒毛高度增加。促肾上腺皮质激素释放因子(CRF)mRNA在回肠中上调0.9倍，而在结肠中不表达。在CS猪的结肠中，Ucn2(Ucn2)基因表达上调1.5倍，而在回肠中则不表达。与对照组相比，回肠和结肠中均观察到促炎症细胞因子mRNA(IL1B、TNFA、IL8和IL6)的下调。相反，CS诱导回肠和结肠中IL10和肥大细胞糜酶基因(CMA1)的mRNA显着上调。综上所述，这些数据表明，猪的慢性应激导致肠道屏障和营养物质运输功能以及神经免疫介质和受体表达的显著变化。
Psychosocial stress is a major factor driving gastrointestinal (GI) pathophysiology and disease susceptibility in humans and animals. The mechanisms governing susceptibility to stress-induced GI disease remain poorly understood. In the present study, we investigated the influence of chronic social stress (CSS) in pigs, induced by 7 d of chronic mixing/crowding stress, on intestinal barrier and nutrient transport function, corticotropin releasing factor (CRF) signaling and immunological responses. Results from this study showed that CSS resulted in a significant impairment of ileal and colonic barrier function indicated by reduced transepithelial electrical resistance (TER) in the ileum and increased FD4 flux in the ileum (by 0.8 fold) and colon (by 0.7 fold). Ileal sodium glucose linked transporter 1 (SGLT-1) function, measured as glucose-induced changes in short-circuit current (Isc), was diminished (by 52%) in CSS pigs, associated with reduced body weight gain and feed efficiency. Although reductions in SGLT-1 function were observed in CSS pigs, mRNA expression for SGLT-1, villus heights were increased in CSS pigs. Corticotropin releasing factor (CRF) mRNA was upregulated (by 0.9 fold) in the ileum of CSS pigs but not in the colon. Urocortin 2 (Ucn2) mRNA was upregulated (by 1.5 fold) in the colon of CSS pigs, but not in the ileum. In CSS pigs, a downregulation of pro-inflammatory cytokines mRNA (IL1B, TNFA, IL8, and IL6) was observed in both ileum and colon, compared with controls. In contrast CSS induced a marked upregulation of mRNA for IL10 and mast cell chymase gene (CMA1) in the ileum and colon. Together, these data demonstrate that chronic stress in pigs results in significant alterations in intestinal barrier and nutrient transport function and neuro-immune mediator and receptor expression.