Self-synthesized extracellular matrix contributes to mature adipose tissue regeneration in a tissue engineering chamber
Self-synthesized extracellular matrix contributes to mature adipose tissue regeneration in a tissue engineering chamber
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自合成的细胞外基质有助于组织工程室中成熟脂肪组织的再生
DOI：
10.1111/wrr.12292
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发表时间：
2015
影响因子：
2.9
通讯作者：
Lu Feng
中科院分区：
文献类型：
--
作者：
Zhan Weiqing;Chang Qiang;Xiao Xiaolian;Dong Ziqing;Zeng Zhaowei;Gao Jianhua;Lu Feng
开发一种能够支持可靠、可预测和完全的脂肪组织再生的工程化脂肪组织替代物将在整形和重建外科中具有价值。对于脂肪生成，组织工程室提供了一个优化的微环境，既有效又可重复；然而，由于尚不清楚的原因，在组织工程室中再生的组织主要由结缔组织组成，而不是脂肪组织。在这里，我们描述了一种基于小室的系统，用于提高成熟脂肪组织的产量，并讨论了组织小室模型中脂肪生成的潜在机制。将带独立血管蒂的脂肪组织瓣植入兔体内。脂肪体积在观察期间(1、2、3、4、16周)显著增加。组织形态计量学显示成熟的脂肪组织有脂肪组织重塑的迹象。诱导的工程化构建物高水平表达成脂基因(γ)、趋化基因(基质细胞衍生因子1a)和炎性基因(白介素1和6)。在我们的系统中，细胞外基质可能作为细胞迁移和增殖的支架，允许在室内微环境中获得成熟的脂肪组织，而不需要外源支架。我们的结果为脂肪组织再生早期发展中的关键因素提供了新的见解。
The development of an engineered adipose tissue substitute capable of supporting reliable, predictable, and complete fat tissue regeneration would be of value in plastic and reconstructive surgery. For adipogenesis, a tissue engineering chamber provides an optimized microenvironment that is both efficacious and reproducible; however, for reasons that remain unclear, tissues regenerated in a tissue engineering chamber consist mostly of connective rather than adipose tissue. Here, we describe a chamber‐based system for improving the yield of mature adipose tissue and discuss the potential mechanism of adipogenesis in tissue‐chamber models. Adipose tissue flaps with independent vascular pedicles placed in chambers were implanted into rabbits. Adipose volume increased significantly during the observation period (week 1, 2, 3, 4, 16). Histomorphometry revealed mature adipose tissue with signs of adipose tissue remolding. The induced engineered constructs showed high‐level expression of adipogenic (peroxisome proliferator‐activated receptor γ), chemotactic (stromal cell‐derived factor 1a), and inflammatory (interleukin 1 and 6) genes. In our system, the extracellular matrix may have served as a scaffold for cell migration and proliferation, allowing mature adipose tissue to be obtained in a chamber microenvironment without the need for an exogenous scaffold. Our results provide new insights into key elements involved in the early development of adipose tissue regeneration.