From liposomes to supported, planar bilayer structures on hydrophilic and hydrophobic surfaces:: An atomic force microscopy study
From liposomes to supported, planar bilayer structures on hydrophilic and hydrophobic surfaces:: An atomic force microscopy study
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DOI：
10.1016/s0006-3495(00)76549-0
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发表时间：
2000-12-01
影响因子：
3.4
通讯作者：
Puu, G
中科院分区：
文献类型：
--
作者：
Jass, J;Tjärnhage, T;Puu, G
通过在液体中敲击模式原子力显微镜，原位观察了亲水和疏水固体载体上从附着的脂质体到双层贴片的转变过程。以一种平滑的方式，附着的脂质体从外缘向中心铺展和扁平，直到两层膜双层堆叠在一起。然后，顶部双层在底部双层上滚动或滑动，相邻的边缘结合在一起形成更大的膜片。从现场测量的单双层的表观高度6.0-7.5 nm可以清楚地看到这一点。钙的添加似乎增加了阻止显示中间阶段的过程的速度。同样的中间步骤似乎存在于疏水表面，尽管附着的脂质体似乎扭曲了，所得到的膜边缘不平坦。这项工作提供了关于脂质体在固体载体上结合(融合)的直观和详细的信息，并展示了如何使用原子力显微镜来研究这一过程。
The sequence of events involved in the transition from attached liposomes to bilayer patches on hydrophilic and hydrophobic solid supports were visualized in situ by Tapping Mode atomic force microscopy in liquid. In a smooth manner, the attached liposomes spread and flattened from the outer edges toward the center until the two membrane bilayers were stacked on top of each other. The top bilayer then either rolls or slides over the bottom bilayer, and the adjacent edges join to form a larger membrane patch. This is clearly visible from the apparent height of 6.0-7.5 nm of the single bilayer, measured in situ. The addition of calcium appeared to increase the rate of the processes preventing the visualization of the intermediate stages. The same intermediate steps appeared to be present on hydrophobic surfaces, although the attached liposomes seemed to be distorted and the resultant membrane edges were uneven. This work has provided visual and detailed information on liposome coalescence (fusion) onto solid supports and demonstrated how the atomic force microscope can be used to study the process.