Production of human immunoglobulin E antibody in vitro.
Production of human immunoglobulin E antibody in vitro.
复制标题
人免疫球蛋白E抗体的体外生产。
作者：
A. H. Tjio;W. Hull;G. Gleich
免疫球蛋白E(IgE)(1)的发现极大地促进了对反应性抗体(2)介导的超敏反应的理解，并导致了过敏学领域新的诊断方法的发展(3，4)。最近的研究证实了外周血淋巴细胞在体外产生IgE蛋白，并研究了IgE生物合成的动力学和药物控制(5，6)。本次交流的目的是描述我们在体外观察特应性和非特应性个体外周血单个核细胞产生IgE蛋白和抗原特异性IgE抗体的情况。
Waldmann等人淋巴细胞培养方法的改进。(7)在我们的研究中使用。取自11例患者的血，60ml，其中8例为特应性(几何平均血清IgE为247 ng/ml，范围61~1079)，3例为非特应性(几何平均血清IgE为96 ng/ml，范围73~143)。
The discovery of immunoglobulin E (IgE) (1) has contributed significantly to the understanding of hypersensitivity reactions mediated by reaginic antibody (2) and has led to the development of new diagnostic procedures in the field of allergology (3, 4). Recent studies have demonstrated the production of IgE protein by peripheral blood lymphocytes in vitro and have examined the kinetics and pharmacologic control of IgE biosynthesis (5, 6). The purpose of this communication is to describe our observation of in vitro production of IgE protein and antigen-specific IgE antibodies by peripheral blood mononuclear cells obtained from atopic and nonatopic individuals.
A modification of the lymphocyte culture method of Waldmann et al. (7) was used in our studies. Blood, 60 ml, was obtained from 11 patients, eight of whom are atopic (geometric mean serum IgE, 247 ng/ml; range 61 to 1079) and three of whom are nonatopic (geometric mean serum IgE, 96 ng/ml; range 73 to 143).