Comparison of GC-MS and NMR for Metabolite Profiling of Rice Subjected to Submergence Stress
Comparison of GC-MS and NMR for Metabolite Profiling of Rice Subjected to Submergence Stress
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DOI：
10.1021/pr300953k
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发表时间：
2013-02-01
影响因子：
4.4
通讯作者：
Larive, Cynthia K.
中科院分区：
文献类型：
--
作者：
Barding, Gregory A., Jr.;Beni, Szabolcs;Larive, Cynthia K.
干旱、极端温度和洪水等自然灾害会严重影响农作物生产。了解受这些灾害威胁的作物的代谢反应，可以深入了解影响生存的生物反应机制。在这项研究中，对野生型和耐淹型水稻品种进行了长达3天的淹水胁迫，并允许1天的淹水后恢复，对比分析了GC-MS和H-1 NMR结果。大多数代谢组学研究都是使用单一分析平台进行的。然而，每个平台都有其固有的优点和缺点，这些优点和缺点会影响代谢组的分析范围。在这项工作中，通过使用H-1 NMR和GC-MS对植物胁迫反应进行了更彻底的分析。几种代谢物，如s -甲基蛋氨酸和二肽丙酰甘氨酸，只能通过H-1 NMR进行检测和定量。与本研究中使用的GC-TOF-MS相比，NMR的高动态范围，在一次实验中提供了广泛的代谢物覆盖范围。GC-MS的灵敏度有助于糖、有机酸和氨基酸的定量，其中一些是核磁共振无法检测到的，并为TCA循环的调节提供了额外的见解。H-1 NMR和GC-MS联合提供的代谢信息对于了解水稻对淹水的复杂生化和分子响应至关重要。
Natural disasters such as drought, extreme temperatures, and flooding can severely impact crop production. Understanding the metabolic response of crops threatened with these disasters provides insights into biological response mechanisms that can influence survival. In this study, a comparative analysis of GC-MS and H-1 NMR results was conducted for wild-type and tolerant rice varieties stressed by up to 3 days of submergence and allowed 1 day of postsubmergence recovery. Most metabolomics studies are conducted using a single analytical platform. Each platform, however, has inherent advantages and disadvantages that can influence the analytical coverage of the metabolome. In this work, a more thorough analysis of the plant stress response was possible through the use of both H-1 NMR and GC-MS. Several metabolites, such as S-methyl methionine and the dipeptide alanylglycine, were only detected and quantified by H-1 NMR The high dynamic range of NMR, as compared with that of the GC-TOF-MS used in this study, provided broad coverage of the metabolome in a single experiment. The sensitivity of GC-MS facilitated the quantitation of sugars, organic acids, and amino acids, some of which were not detected by NMR, and provided additional insights into the regulation of the TCA cycle. The combined metabolic information provided by H-1 NMR and GC-MS was essential for understanding the complex biochemical and molecular response of rice plants to submergence.