Patch-clamp 'mapping' of ion channel activity in human sperm reveals regionalisation and co-localisation into mixed clusters.
Patch-clamp 'mapping' of ion channel activity in human sperm reveals regionalisation and co-localisation into mixed clusters.
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DOI：
10.1002/jcp.21153
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发表时间：
2007-12
影响因子：
5.6
通讯作者：
Publicover, S
中科院分区：
文献类型：
--
作者：
Jimenez-Gonzalez, M C;Gu, Y;Kirkman-Brown, J;Barratt, C L R;Publicover, S
离子通道是精子生理和功能的许多方面的关键。应用膜片钳技术研究了人精子头部质膜上离子通道的分布。我们报告，三种类型的活动是常见的赤道和顶体区域的精子头部。其中两个（一个氯离子渗透性阴离子通道显示长期稳定的开口和第二个通道之间闪烁的开放和关闭状态，并依赖于细胞质因子的活动）主要定位于赤道段。第三种类型，非常类似于闪烁的活性，但具有不同的电压灵敏度的Popen更广泛地分布，但在前顶体上检测不到。在前顶体区通道，但表现出非常低的水平的自发活动。精子赤道区通道活性的一个独特特征是共定位成混合簇，大多数斑块没有活性，但“活性”斑块通常含有2种或多种类型的活性（在单个200-300 nM直径斑块中）。我们的结论是，在精子膜中的离子通道显示区域化的类型和活动，并聚集成功能组的通道，可能通过局部膜电位的影响相互作用。（207）
Ion channels are pivotal to many aspects of sperm physiology and function. We have used the patch clamp technique to investigate the distribution of ion channels in the plasma membrane of the head of human spermatozoa. We report that three types of activity are common in the equatorial and acrosomal regions of the sperm head. Two of these (a chloride-permeable anion channel showing long stable openings and a second channel which flickered between open and closed states and was dependent upon cyoplasmic factors for activity) were localised primarily to the equatorial segment. A third type, closely resembling the flickering actvivity but with different voltage sensitivity of Popen was more widely distributed but was not detectable over the anterior acrosome. In the anterior acrosomal area channels were present but showed very low levels of spontaneous activity. A unique feature of channel activity in the sperm equatorial region was co-localisation into mixed clusters, most patches were devoid of activity but ‘active’ patches typically contained 2 or more types of activity (in a single 200-300 nM diameter patch). We conclude that ion channels in the sperm membrane show regionalisation of type and activity and that the channels are clustered into functional groups, possibly interacting through local effects on membrane potential. (207)