2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis.
2,4-D and IAA Amino Acid Conjugates Show Distinct Metabolism in Arabidopsis.
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DOI：
10.1371/journal.pone.0159269
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Novak O
中科院分区：
文献类型：
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作者：
Eyer L;Vain T;Pařízková B;Oklestkova J;Barbez E;Kozubíková H;Pospíšil T;Wierzbicka R;Kleine-Vehn J;Fránek M;Strnad M;Robert S;Novak O
除草剂 2,4-D 具有生长素活性，因此可用作合成且可追踪的类似物来研究生长素相关反应。在这里，我们发现不仅外源 2,4-D，而且其酰胺连接的代谢物 2,4-D-Glu 通过 TIR1/AFB 生长素介导的信号通路对植物生长表现出抑制作用。为了进一步研究 2,4-D 代谢物的转化、特性和活性，我们开发了一种基于离子交换和免疫特异性吸附剂与灵敏液相色谱-质谱法相结合的新型纯化程序。在 2,4-D 处理的样品中，检测到的 2,4-D-Glu 和 2,4-D-Asp 浓度比 2,4-D 水平低 100 倍，这表明 2,4-D 可以在植物中代谢。此外，2,4-D-Asp 和 2,4-D-Glu 被确定为 2,4-D 稳态的可逆形式，可以转化为游离 2,4-D。这项工作为植物发育中生长素作用的新研究铺平了道路。
The herbicide 2,4-D exhibits an auxinic activity and therefore can be used as a synthetic and traceable analog to study auxin-related responses. Here we identified that not only exogenous 2,4-D but also its amide-linked metabolite 2,4-D-Glu displayed an inhibitory effect on plant growth via the TIR1/AFB auxin-mediated signaling pathway. To further investigate 2,4-D metabolite conversion, identity and activity, we have developed a novel purification procedure based on the combination of ion exchange and immuno-specific sorbents combined with a sensitive liquid chromatography-mass spectrometry method. In 2,4-D treated samples, 2,4-D-Glu and 2,4-D-Asp were detected at 100-fold lower concentrations compared to 2,4-D levels, showing that 2,4-D can be metabolized in the plant. Moreover, 2,4-D-Asp and 2,4-D-Glu were identified as reversible forms of 2,4-D homeostasis that can be converted to free 2,4-D. This work paves the way to new studies of auxin action in plant development.