Neurotransmitter profile of saccadic omnipause neurons in nucleus raphe interpositus
Neurotransmitter profile of saccadic omnipause neurons in nucleus raphe interpositus
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中缝间核扫视全停神经元的神经递质谱
DOI：
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发表时间：
1994
影响因子：
5.3
通讯作者：
Reichenberger
中科院分区：
文献类型：
--
作者：
Anja;K. Horn;Jean;A.;Biittner;Petra;Wahle;lngrid;Reichenberger
扫视全停神经元（OPN）对于扫视眼球运动的产生至关重要。在灵长类动物中，OPN位于中缝间位核（rip）内的中线附近。在本研究中，我们使用了几种不同的神经解剖学方法来研究猴子中与OPNs相关的递质。用钙结合蛋白小清蛋白免疫标记猴的OPN，并确定猫和人的同源细胞群。抗GABA、甘氨酸（GLY）、谷氨酸（GLU）、5-羟色胺（5-HT）和酪氨酸羟化酶的抗体的使用显示，OPNs的胞体是GLY免疫反应性的，但它们缺乏GABA和5-HT免疫染色。GAD 67 mRNA探针原位杂交证实了OPN的GABA免疫染色阴性。将~ 3 H-GLY注入OPNs投射野，内侧纵束的嘴侧间质核（riMLF）--垂直扫视爆发神经元区。这导致RIP中OPN的选择性逆行标记，而在上级丘中没有发现标记，其向riMLF发送兴奋性投射。在riMLF中，推测的爆发神经元的胞体和树突被许多GLY免疫反应终末接触。免疫反应性终末样结构接触OPNs的定量分析显示，从GLY和GABA阳性终末的胞体和树突上的强输入，而GLU阳性斑点主要局限于树突。很少有5-HT和儿茶酚胺能终末接触OPN胞体。我们的研究结果表明，OPN使用GLY作为神经递质，它们接受大量来自GABA能、甘氨酸能和多巴胺能传入的接触，而来自单胺能输入的接触明显较少。
Saccadic omnipause neurons (OPNs) are essential for the generation of saccadic eye movements. In primates OPNs are located near the midline within the nucleus raphe interpositus (rip). In the present study we used several different neuroanatomical methods to investigate the transmitters associated with OPNs in the monkey. Immunolabeling for the calcium-binding protein parvalbumin was employed to mark OPNs in the monkey and define the homologous cell group in cat and human. The use of antibodies against GABA, glycine (GLY), glutamate (GLU), serotonin (5-HT), and tyrosine hydroxylase revealed that the somata of OPNs are GLY immunoreactive, but they are devoid of GABA and 5-HT immunostaining. In situ hybridization with the GAD67 mRNA probe confirmed the negative GABA immunostaining of OPNs. 3H-GLY was injected into a projection field of OPNs, the rostral interstitial nucleus of the medial longitudinal fascicle (riMLF)--the vertical saccadic burst neuron area. This resulted in selective retrograde labeling of the OPNs in rip, while no labeling was found in the superior colliculus, which sends an excitatory projection to the riMLF. The somata and dendrites of putative burst neurons in the riMLF were contacted by numerous GLY- immunoreactive terminals. The quantitative analysis of immunoreactive terminal-like structures contacting OPNs revealed a strong input from GLY- and GABA-positive terminals on somata and dendrites, whereas GLU- positive puncta were mainly confined to the dendrites. Very few 5-HT and catecholaminergic terminals contacted OPN somata. Our findings suggest that OPNs use GLY as a neurotransmitter, and they receive numerous contacts from GABAergic, glycinergic, and glutaminergic afferents, and significantly fewer from monoaminergic inputs.
影响因子：
56.9
作者：
KAUFMAN, DL;MCGINNIS, JF;TOBIN, AJ
通讯作者：
TOBIN, AJ
影响因子：
2.5
作者：
SCUDDER, CA;FUCHS, AF;LANGER, TP
通讯作者：
LANGER, TP