Impaired calcium mobilization and differential tyrosine phosphorylation in intestinal intraepithelial lymphocytes.
Impaired calcium mobilization and differential tyrosine phosphorylation in intestinal intraepithelial lymphocytes.
复制标题
肠上皮内淋巴细胞钙动员受损和酪氨酸磷酸化差异。
DOI：
10.1046/j.1365-2567.1997.00228.x
复制
发表时间：
1997
期刊：
影响因子：
6.4
通讯作者：
Mccormack,WT
中科院分区：
文献类型：
--
作者：
Sanchez-Garcia,FJ;Aller,WW;Mccormack,WT
肠上皮内淋巴细胞（iIEL）在体外表现出独特的活化状态，其特征在于活化标志物的表达和效应功能，但对促有丝分裂信号的反应最小。为了进一步表征这种活化状态，将iIEL与脾T细胞的两个关键活化信号（钙动员和酪氨酸磷酸化）进行比较。 在用钙离子载体离子霉素或A23187、毒胡萝卜素或通过CD 3交联处理的iIEL中，钙动员受损。成熟和胚胎iIEL都存在钙动员缺陷。抗磷酸酪氨酸蛋白质印迹分析显示，iIEL能够通过酪氨酸磷酸化响应T细胞受体（TCR）介导的信号，尽管磷酸化模式与脾T细胞中观察到的不同。 我们得出结论，iIEL无法在体外动员钙，这可能是由于肠上皮微环境对TCR介导的信号转导途径的调节和/或由用于制备iIEL的标准分离程序引起的，这在未来iIEL功能的体外研究中必须考虑。
Intestinal intraepithelial lymphocytes (iIEL) exhibit a unique activation state characterized by the expression of activation markers and effector functions, but a minimal response to mitogenic signalsin vitro. To further characterize this activation status, iIEL were compared with splenic T cells for two key activation signals, calcium mobilization and tyrosine phosphorylation. Calcium mobilization was impaired in iIEL treated with the calcium ionophores ionomycin or A23187, thapsigargin, or by CD3‐cross‐linking. The calcium mobilization defect is shared by mature and embryonic iIEL. Anti‐phosphotyrosine Western blot analysis revealed that the iIEL are able to respond to T‐cell receptor (TCR)‐mediated signals by tyrosine phosphorylation, although the patterns of phosphorylation differ from those seen in splenic T cells. We conclude that iIEL are unable to mobilize calciumin vitro, which may be due to modulation of TCR‐mediated signal transduction pathways by the microenvironment of the intestinal epithelium and/or caused by the standard isolation procedure used to prepare iIEL, which must be considered in futurein vitrostudies of iIEL function.